洋葱高频离体再生体系的建立  

Establishment of High-frequency in Vitro Regeneration System of Onion

作　　者：王建军[1] 刘照坤[1] 侯喜林[1] 杨学东[1] 孔敏[1] 李满堂[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,农业部华东地区园艺作物生物学与种质创制重点实验室,南京210095

出　　处：《园艺学报》2012年第7期1380-1386,共7页Acta Horticulturae Sinica

基　　金：国家公益性行业(农业)科研专项(200903018)

摘　　要：以洋葱(Allium cepa L.)品种‘W465’的幼嫩花序和不同发育时期的幼蕾为外植体材料,研究激素配比对其愈伤组织诱导、再生和生根驯化的影响,以建立洋葱高频离体再生体系。研究结果表明,洋葱幼嫩花序为最佳外植体材料,在含有1.0mg.L-12,4-D+1.0mg.L-16-BA的B5培养基上愈伤诱导率为100%,在添加1.0mg.L-1TDZ的B5培养基上再生芽分化率为78.13%,在1/2MS+0.01mg.L-1NAA培养基上可诱导生根,驯化移栽成活率达到95%。With the young umbels and buds of onion （Allium cepa L.） at different development stage, a high frequency regeneration system of onion in vitro was established by analysis the effects of explant type and plant growth regulator combination on callus＇ induction, shoot regeneration and rooting. The optimum condition was showed as with young umbels on B5 medium with 1.0 mg. L^-1 2,4-D ＋ 1.0mg. L^-1 6-BA, with the callus induction rate reaching 100%. The optimum hormone concentration of getting high-frequency regeneracy shoot, with 78.13%, from callus was 1.0 mg·L^-1 thidiazuron （TDZ） on B5 medium. All shoots rooted on 1/2MS medium with 0.01 mg · L^-1 NAA, the regenerated plantlet were domesticated and transplanted in greenhouse： The survival rate was 95%.

关 键 词：洋葱 幼嫩花序 组织培养 再生 苯基噻二唑脲(TDZ) 

分 类 号：S633.2[农业科学—蔬菜学]