芒果甙对柔红霉素致大鼠心肌毒性细胞中铁调节蛋白表达的影响  

EFFECTS OF MANGIFERIN ON IRON REGULATORY PROTEIN EXPRESSION OF DAUNORUBICIN-INDUCED RAT MYOCARDIAL TOXICITY CELLS

作　　者：石泽延[1] 彭志刚[1] 马劼[1] 赵卫华[1] 杨杰[1] 姚弈斌[1] 吴泽广[1] 唐涌连[1] 

机构地区：[1]广西医科大学第一附属医院血液内科,南宁530021

出　　处：《广西医科大学学报》2012年第2期169-171,共3页Journal of Guangxi Medical University

基　　金：广西自然科学基金资助项目(合同号:2010GXNSFA013177)

摘　　要：目的:探讨铁调节蛋白1(IRP1)及铁调节蛋白2(IRP2)在芒果甙保护柔红霉素致大鼠心肌细胞毒性作用中的表达及其意义。方法:以4μmol/L柔红霉素单用或联合应用25～200μmol/L芒果甙为处理因素作用于大鼠心肌细胞24,48h及72h,应用qRT-PCR法检测各组心肌细胞IRP1、IRP2mRNA表达。结果:25～200μmol/L芒果甙模型组在干预24h后IRP1、IRP2表达水平较柔红霉素组(4μmol/L)降低,干预48,72h后,二者表达较柔红霉素组升高。结论:芒果甙能改变柔红霉素致大鼠心肌毒细胞中铁调节蛋白的表达水平,对细胞内铁代谢产生影响。Objective：To study the effects of Mangiferin on iron regulatory protein 1（IRP1） and iron regulatory protein 2（IRP2） expression and significance of daunorubicin-induced rat myocardial cell toxicity in vitro.Methods： Rat myocardial cells were treated with 4 μmol/L daunorubicin alone or combined application of 25~200 μmol/L concentrations of mangiferin for 24,48 and 72 hours,respectively.The mRNA expression of IRP1 and IRP2 in myocardial cells was detected by qRT-PCR assay.Results： The expression of IRP1 and IRP2 was lower in mangiferin model groups（25~200 μmol/L） than in negative control group at 24 h,whereas the expression of IRP1 and IRP2 was higher in mangiferin model groups than in daunorubicin group（4 μmol/L） at 48 and 72 hours.Conclusion： Mangiferin can change the iron regulatory protein expression of daunorubicin-induced rat myocardial toxicity cells,then affect intracellular iron metabolism.

关 键 词：铁调节蛋白 芒果甙 柔红霉素 大鼠心肌细胞 

分 类 号：R969[医药卫生—药理学]