马尾松树皮提取物抗肿瘤作用的实验研究  

THE ANTITUMOR ROLE OF PINUS MASSONIANA BARK EXTRACT

作　　者：张锦宏[1] 冯冬茹[2] 刘兵[2] 戚康标[2] 刘炎明[2] 王宏斌[2] 谢衡 李若达 王金发[2] 

机构地区：[1]广州医学院医学遗传学与细胞生物学教研室,广州510182 [2]中山大学生科院生物防治和基因工程教育部重点实验室,广州510275 [3]广州嵩珍营养源研究所,广州510645

出　　处：《广西医科大学学报》2012年第2期179-182,共4页Journal of Guangxi Medical University

基　　金：广东省工业攻关项目(No.2004B10401033);广州医学院科研基金(No.04-K-19;0115-038111)资助

摘　　要：目的:探讨马尾松树皮提取物(Pinus massoniana bark extract,PMBE)对S180腹水瘤小鼠的抗肿瘤作用。方法:采用NIH小鼠S180腹水瘤模型,通过腹腔注射PMBE,观察PMBE对S180腹水瘤小鼠的体重增长、腹水瘤体积、抑瘤率、胸腺和脾脏的脏器系数、外周血白细胞数量、T淋巴细胞增殖率、凋亡率和细胞周期的变化。结果:PMBE对S180腹水瘤细胞表现出明显抑制作用。50,100,200 mg/kgPMBE剂量组的抑瘤率分别为9.95%、65.74%、88.04%;小鼠的体重增长相应下降;PMBE高剂量组能明显提高胸腺指数、脾脏指数、外周血白细胞数量和T淋巴细胞增殖率。PMBE中剂量组能明显诱导S180细胞凋亡,且细胞周期分析显示PMBE使S180细胞阻滞在S期。结论:PMBE通过增强免疫功能和诱导细胞凋亡来抑制S180腹水瘤生长。Objective：To investigate the antitumor activity of Pinus massoniana bark extract（PMBE） in S180 ascites tumor-bearing mice.Methods： After the intraperitoneal injection of PMBE in the S180 ascities tumor-bearing NIH mice,the changes of the body weight,ascites tumor size,tumor inhibition rate,thymus index,spleen index,peripheral leucocyte count and T lymphocyte proliferation rate,apoptosis rate and cell cycle were observed.Results： PMBE showed significantly inhibition on the growth of S180 ascites tumor cells.The inhibitory rates of PMBE were 9.95%,65.74% and 88.04% at the dosages of 50,100,200 mg/kg,respectively;a corresponding decline in weight gain of the mice.The dose of 200 mg/kg PMBE could increase the thymus index,spleen index,peripheral leucocyte count and T lymphocyte proliferation rate.The dose of 100 mg/kg PMBE could induce significantly S180 cell apoptosis,and the cell cycle analysis showed that PMBE induces cell cycle arrest in the S phase.Conclusion： PMBE could inhibit the growth of S180 ascites tumor by increasing immune function and inducing cell apoptosis.

关 键 词：PMBE S180 免疫功能 凋亡 

分 类 号：R969[医药卫生—药理学]