左旋—二脱水伊地醇双甲磺酸酯对HL-60细胞增殖和凋亡的影响  

EFFECTS OF DMDAI-L ON PROLIFERATION INHIBITION AND APOPTOSIS IN HL-60 CELLS

作　　者：曾春[1] 郭超[1] 石玲东[1] 刘元[2] 曹斌[2] 杨斌[1] 

机构地区：[1]广西医科大学药学院,南宁530021 [2]广西中医药研究院,南宁530022

出　　处：《广西医科大学学报》2012年第2期183-186,共4页Journal of Guangxi Medical University

基　　金：广西自然科学基金项目应用基础研究专项基金资助(No.桂科基0991013)

摘　　要：目的:研究左旋—二脱水伊地醇双甲磺酸酯(DMDAI-L)对HL-60细胞增殖和凋亡的影响。方法:采用CCK-8法检测不同浓度的DMDAI-L对HL-60细胞的增殖抑制活性;通过流式细胞术PI单染法分析周期变化;Annexin V/FITC-PI法检测细胞凋亡。结果:DMDAI-L对HL-60细胞增殖具有良好的抑制作用,并呈浓度时间依赖关系,DMDAI-L作用于HL-60细胞24,48,72h的IC50分别为(57.514±2.089)μg/mL、(5.234±0.754)μg/mL、(2.451±0.092)μg/mL;流式细胞仪显示,HL-60细胞凋亡率随DMDAI-L浓度升高而增高,且细胞周期被阻滞于G1/G0期。结论:DMDAI-L在体外对HL-60细胞有很强的抑制作用,可能是通过对HL-60细胞的G1/G0期阻滞和凋亡诱导作用来实现增殖抑制的目的。Objective：To investigate the effects of DMDAI-L on proliferation inhibition and apoptosis in HL-60 cells.Methods： CCK-8 assay was used for evaluating the inhibition of proliferation on HL-60 cells which treated with different concentrations of DMDAI-L;PI staining assay detected the cycle change of HL-60 cells in flow cytometry;the apoptotic cells were analyzed by FCM using Annexin V FITC-PI double staining method.Results： DMDAI-L could inhibit the proliferation of HL-60 cells in a dose-and time-dependent manner,the IC50 value of DMDAI-L for 24 h,48 h and 72 h were（57.514±2.089）,（5.234±0.754） and（2.451±0.092） μg/mL;respectively.Flow cytometric analysis showed that with the increase of DMDAI-L concentration,the apoptosis rate of HL-60 cells increased and cell cycle was arrested at G1/G0 phase.Conclusion： DMDAI-L has strong effects on HL-60 cells in vitro,it suggests that DMDAI-L could inhibit the proliferation of HL-60 cells possibly through G1/G0 phase arrested and induction of apoptosis.

关 键 词：DMDAI-L HL-60细胞 细胞增殖抑制 细胞凋亡 周期阻滞 

分 类 号：R969[医药卫生—药理学]