抗苗勒管激素及其Ⅱ型受体基因多态性与卵巢储备功能的关系  

Association between Gene Polymorphism of Anti-Mullerian Hormone and Its Type Ⅱ Receptor and Ovarian Reserve

作　　者：田春花[1] 胡蓉[2] 贾韶彤[2] 兰生学 田进石 王飞苗[2] 李彩艳[2] 杨海燕[2] 姜银芝[2] 

机构地区：[1]宁夏医科大学研究生院,银川750001 [2]宁夏医科大学附属总医院生殖医学中心,宁夏医科大学生育力保持省部共建教育部重点实验室,银川750001 [3]宁夏吴忠市人民医院中西医结合科,吴忠751100 [4]宁夏吴忠市人民医院急救中心,吴忠751100

出　　处：《华中科技大学学报（医学版）》2012年第3期315-319,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：宁夏自然科学基金资助项目(No.NZ10115);宁夏自治区科技攻关项目(No.201045);银川市2010年第一批科技项目(No.2010452);宁夏医科大学“博士学位建设学科”开放课题(KF2010-49);宁夏高等学校科研项目(No.2010297)

摘　　要：目的探讨抗苗勒管激素(AMH)及其Ⅱ型受体(AMHRⅡ)基因多态性与卵巢储备功能的相关性。方法采用聚合酶链反应(PCR)及DNA测序方法,检测94例多囊卵巢综合征(PCOS)患者、94例正常排卵女性及32例卵巢功能早衰(POF)患者AMH和AMHRⅡ基因型及等位基因频率的分布情况,分析患者AMH、AMHRⅡ基因型不同与其年龄、体重指数(BMI)及激素水平的相关性。结果①3组研究对象AMH、AMHRⅡ基因型及AMH等位基因频率分布存在差异(均P<0.05),而AMHRⅡ等位基因频率分布无差异(均P>0.05);②PCOS患者AMH基因型与BMI、LH、T水平相关(均P<0.05),而正常对照组和POF患者AMH基因型与年龄、BMI、FSH、LH、T、E2、PRL水平无关(均P>0.05);③POF患者AMHR基因型与BMI、T及E2水平相关(均P<0.05),而正常组和PCOS患者AMHRⅡ基因型分布与其年龄、BMI、FSH、LH、T、E2、PRL水平均无关(均P>0.05)。结论 AMH及AMHRⅡ基因的多态性可能与卵巢储备功能异常有关。Objective To investigate gene polymorphism of the anti-Mullerian hormone（AMH）and its type Ⅱ receptor（AMHRⅡ）in polycystic ovary syndrome（PCOS）and premature ovarian failure（POF）.Methods Polymerase chain reaction（PCR）and DNA sequencing were used to detect the distribution of AMH and AMHRⅡ genotype and allele frequency in 94 cases of PCOS,94 normal women and 32 cases of POF.The correlation between AMH and AMHRⅡ genotypes with age,body mass index（BMI）and hormone levels was analyzed.Results ① There was significant difference in AMH and AMHRⅡ genotypes,and AMH allele frequency among three groups of subjects（P0.05）,but there was no significant difference in AMHRⅡ allele frequency distribution（P0.05）;② In PCOS patients,AMH genotype was correlated with BMI,LH,T levels（P0.05）,while in the normal group and POF group,AMH genotypes were not associated with age,BMI,FSH,LH,T,E2,PRL（P0.05）;③ In POF patients,AMHRⅡ genotypes were associated with BMI,T and E2（P0.05）,while in the normal group and PCOS group,AMHRⅡ genotypes were not associated with age,BMI,FSH,LH,T,E2,PRL（P0.05）.Conclusion AMH and AMHRⅡ gene polymorphism may be associated with PCOS,POF.

关 键 词：抗苗勒管激素 抗苗勒管激素Ⅱ型受体 基因多态性 多囊卵巢综合征 卵巢功能早衰 

分 类 号：R711.6[医药卫生—妇产科学]