石龙芮毛茛愈伤组织诱导及再生体系建立研究  

The study on callus inducement and establishment of regeneration clone of Ranunculus sceleratus

作　　者：李洁[1] 姜朋[1] 吴丽巍[1] 张冬艳[1] 姜长阳[1] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116081

出　　处：《中国园艺文摘》2012年第6期24-26,31,共4页Chinese Horticulture Abstracts

基　　金：辽宁省高等教育教学改革资助项目(20090304);辽宁师范大学教学改革资助项目(LSJG:20090108)

摘　　要：为保护野生资源、实现人工栽培,以石龙芮毛茛的下胚轴为材料,进行愈伤组织的诱导和分化、不定芽分化、试管苗生根和生根继代、试管苗移栽和定植的研究,建立起石龙芮毛茛的下胚轴再生体系。结果表明:MS+KT0.4mg/L+2,4-D1.8mg/L是愈伤组织诱导培养和增殖继代培养的理想培养基;MS0是愈伤组织分化培养的理想培养基;采用向培养瓶中加入约5ml浓度为2mg/L的NAA溶液处理24h,再把不定芽切下接种到1/3MS+IAA0.3mg/L培养基的方法,是不定芽生根培养和生根继代培增殖培养的理想方法。试管苗移栽、定植成活率较高;定植的试管苗保持石龙芮毛茛的所有植物学性状。In order to preserve the wild resources and achieve planting, hypocotyls of Ranunculus sceleratus were used as material to do the research on callus inducement and differentiation, differentiation of adventitious buds, rooting and rooting multiplication of tube seedlings, transplantation and stable planting of tube seedlings, then the regeneration clone of Ranunculus sceleratus with hypocotyls was established. The results showed that the ideal medium for callus inducing and callus multiplication was MS＋KT 0.4 mg/L＋2,4-D 1.8 mg/L; MS 0 was the best medium for callus differentiation; cultivated in the mediums 1/3MS＋ IAA0.3 mg/L after the adventitious buds disposed 24 hours with 5 ml NAA2 mg/L was the best method for rooting of adventitious buds and rooting multiplication. The rate of transplantation and stable planting of tube seedlings were high, and the tube seedlings stable planted maintained all botany characteristics of the Ranunculus sceleratus.

关 键 词：石龙芮毛茛 愈伤组织 再生体系 

分 类 号：S567.239[农业科学—中草药栽培]