小鼠骨髓染色体G显带最佳制备方法的探究  

Study on the Best Preparation Method of Chromosome of G-banding in Mice Marrow Cell

作　　者：刘俊[1] 李栋[1] 张亚龙[1] 徐艳岩[1] 

机构地区：[1]滨州医学院药学院,山东烟台264003

出　　处：《畜牧与饲料科学》2012年第5期21-22,共2页Animal Husbandry and Feed Science

基　　金：滨州医学院大学生科技创新活动基金项目(BY2011DKCX84)

摘　　要：[目的]介绍一种改进的染色体G显带标本制备技术。[方法]采用小鼠骨髓细胞,改进取材方法、玻片的制备、胰酶处理时间以及染色方法等关键步骤,获得了染色体标本制备的最佳处理条件和试验方法。[结果]获得了更多的分散较好的分裂相,建立了一套实用性强、带型清晰、结果稳定、简单易行的染色体G显带制片技术。[结论]结果为规范小鼠染色体标本的制备方法,提高实验教学效果提供了参考。[Objective] An improved preparation technology of G-banding chromosome sample was introduced. [Method] Mouse bone marrow cells were used, some key steps such as pancreatic enzyme, staining methods were improved, and the best experimental condition and methods were obtained. [Result] More and better scattered mitosis figures were obtained, and a high practical, simple and easy preparation technology of chromosome of G-banding with clear banding pattern and stable results was established. [Conclusion] The research provides a practical reference for the specification of mice chromosome specimen preparation methods, and improves the experimental teaching effect.

关 键 词：骨髓细胞 染色体标本 G显带 GIEMSA染色 

分 类 号：R55[医药卫生—血液循环系统疾病]