内源性绵羊肺腺瘤病毒全基因组序列测定与序列分析  

Cloning and sequence analysis of endogenous jaagsiekte sheep retrovirus

作　　者：周艳喜[1] 苏佳[1] 李靖[1] 刘霄卉[1] 马学恩[1] 

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018

出　　处：《中国兽医学报》2012年第7期962-966,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31060332)

摘　　要：参照内源性绵羊肺腺瘤病毒株enJSRV-20全基因组序列设计合成3对引物,应用PCR技术扩增内源性绵羊肺腺瘤病毒基因片段分别连接pMD-18T载体并测序,完成了内源性绵羊反转录病毒基因组序列测定。分析结果显示,测定序列全长7 519bp,与内源性绵羊肺腺瘤病毒代表株enJSRV-20核苷酸同源性为99.4%,与外源性绵羊肺腺瘤病毒代表株AF357971.1的核苷酸同源性为90.4%。基于全基因组序列核苷酸的系统进化发生树分析结果显示,扩增序列与enJSRV-20的亲缘关系最近。Three pair of primers were designed according to the sequence of enJSRV-20 strain. Sequence of enJSRV was amplified by PCR technology and the PCR product was cloned into pMD18-T vector and then sequenced. So complete sequence of enjSRV was analyzed. Analysis results show that the sequences of enJSRV-20 strain had 99. 4% similarity with enJSRV sequenced, the similarity with AF357971. 1 strain was 90. 4%. Phylogenetic analysis based complete sequence showed that the sequence had the closest linkage to enJSRV-20. It will be useful in the study of infectivity clone and the function of enJSRV.

关 键 词：内源性绵羊肺腺瘤病毒 PCR扩增 全基因序列 

分 类 号：S852.65[农业科学—基础兽医学]