大熊猫血液标本放置时间对全血细胞计数的影响  

Effect of storage time for the blood samples on the complete blood cell count in giant pandas

作　　者：邓林华[1,2] 李德生[1] 张和民[1] 王承东[1] 汤纯香[1] 黄炎[1] 颜其贵[2] 严玉宝[3] 王晓军[1] 

机构地区：[1]中国保护大熊猫研究中心,四川卧龙623006 [2]四川农业大学动物医学院,四川雅安625104 [3]四川出入境检验检疫局,成都610000

出　　处：《黑龙江畜牧兽医》2012年第7期145-146,共2页Heilongjiang Animal Science And veterinary Medicine

基　　金：科技部质检公益项目(200910188)

摘　　要：为了探讨血液标本放置时间对大熊猫全血细胞计数的影响,试验采用平行试验的方法测定了28只健康成年大熊猫静脉血在120 min内放置不同时间时,红细胞(RBC)、血红蛋白(HGB)、白细胞(WBC)、血小板(PLT)、红细胞比容(HCT)平均值的差异。结果表明:血液标本放置60分钟时RBC、HGB、WBC基本无变化,与即检结果相比差异不显著(P>0.05),放置15,30,45,90,120分钟时与即检结果相比差异显著(P<0.05)。PLT随着放置时间的延长有上升趋势,但差异不显著(P>0.05)。HCT在血液标本放置30 min以内均无显著差异(P>0.05),放置45 min后均呈显著性差异(P<0.05)。综合分析各项细胞计数测定结果认为,经乙二胺四乙酸二钾(EDTA-K2)抗凝的大熊猫全血标本,若采血后不能及时检测,应在放置60 min后再进行测定,可提高结果的准确性。To investigate the effect of the blood samples on the complete blood cell count in giant pandas,twenty-eight venous blood samples of the healthy adult giant pandas were used to determine the average differences in the red blood cell（RBC）,hemoglobin（HGB）,white blood cell（WBC）,platelet（PLT）,hematocrit（HCT） when the blood samples were stored for different time in 120 min.The results showed that there was almost no change（P0.05） in the levels of when the blood samples were stored for 60 min;there were significant differences（P0.05） when the blood samples were stored for 15 min,30 min,45 min,90 min and 120 min.PLT had a rising trend due to the effect of the storage time,but there was no significant difference（P0.05）.There was no difference（P0.05） in the level of HCT when the blood samples were stored in 30 min,but there were significant differences（P0.05） when the blood samples were stored after 45 min.It is concludes that if the whole blood samples anticoagulated with EDTA-K2 aren＇t detected in time after blood collection,they should be stored for 60 min and then were determined to improve the accuracy of the results.

关 键 词：大熊猫 血液标本 放置时间 全血细胞计数 

分 类 号：S864.1[农业科学—野生动物驯养]