丁酸钠逆转H460/5-FU肺癌细胞耐药性的研究  

Study on the Drug Resistance Reversal of Lung Cancer Cells H460/5-FU by Sodium Butyrate

作　　者：吴欣欣[1] 李晓艳[1] 武虎[1] 刘海燕[1] 陈侃[1] 

机构地区：[1]浙江理工大学生命科学学院新元医学与生物技术研究所,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2012年第4期609-613,共5页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：国家自然科学基金(30801379);浙江理工大学科研启动基金(1016834-Y);浙江省重中之重学科开放基金项目(SWYX0812)

摘　　要：肺癌耐药是化疗成功的主要障碍,因此肺癌细胞的耐药逆转研究具重要意义。实验通过流式细胞术检测到对5-氟脲嘧啶(5-Fluorouracil,5-FU)耐受的细胞系H460/5-FU中的SP细胞含量较亲本细胞H460明显升高,经丁酸钠诱导分化后,H460/5-FU对5-FU的敏感性增加,表现为细胞存活率降低,细胞凋亡率提高,而且这种改变随着丁酸钠浓度的增加而增强。结果提示丁酸钠具备逆转肺癌耐药细胞系H460/5-FU耐药的能力。The drug resistance of lung cancer is a major obstacle to chemotherapy, so it＇s significant to study the drug resistance reversal of lung cancer cells. This experiment detected the content of SP cells in 5-FU-resistant cell line H460/5-FU through flow cytometry, results showed the content of SP cells in H460/5-FU was much more than that in its parental cell H460, after induction of differentiation by sodium butyrate, the cell sensitivity of H460/5-FU to 5-FU increased, the survival of cells decreased, the rate of cell apoptosis increased, and this change enhanced with the concentration increasing of sodium butyrate. These results prompted sodium butyrate had the ability to reverse the drug resistance of drug-resistant lung cancer cell lines H460/5-FU.

关 键 词：肺癌 丁酸钠 SP细胞 耐药逆转 

分 类 号：Q733.7[生物学—分子生物学]