仔猪圆环病毒2型与高致病性猪繁殖与呼吸综合征病毒混合感染的诊断与病毒变异分析  

Detection of co-infection of Porcine circovirus 2 and high-pathogenic PRRSV and analysis of viral variation

作　　者：肖跃强[1,2] 刘吉山[1,2] 吴忆春[3] 李峰[1,2] 吕素芳 魏凤[1,2] 沈志强[1,2] 

机构地区：[1]山东省滨州畜牧兽医研究院,山东滨州256600 [2]山东绿都生物科技有限公司,山东滨州256600 [3]山东滨州职业学院生物工程学院,山东滨州256603

出　　处：《中国兽医杂志》2012年第6期21-25,共5页Chinese Journal of Veterinary Medicine

基　　金：山东省自然科学基金项目(Y2006D26)

摘　　要：2010年,山东某规模化猪场2月龄仔猪群暴发未知疫病,发病率在80%以上、死亡率在50%以上。结合临床症状、剖检病变,以及PCR或RT-PCR检测,证实该猪场发生PCV-2与高致病性PRRSV混合感染。并且细菌病排查证实还混合感染了猪肺疫。通过对感染的PCV-2ORF2基因、PRRSV Nsp2基因部分序列进行分析显示,PCV-2毒株进化来源差异较大,毒株间核苷酸序列同源性仅为94.2%,但均属于2b基因型,在我国均广泛流行;高致病性PRRSV毒株序列同源性在99.7%以上,说明毒株进化来源同一,并且与近期报道毒株序列的同源性在99%以上,而与2006年分离毒株的序列同源性在95.8%～97.2%之间。以上结果表明,该猪场感染了不同进化来源的PCV-2,所感染的高致病性PRRSV高度同源,但相比2006年报道毒株已经发生一定程度的变异。The outbreak of an unknown disease among piglets resulted in more than 80% morbidity and 50% mortality in a large-scale pig industry in Shandong province.The co-infection of PCV-2 and high-pathogenic PRRSV were identified according to the clinical symptoms,pathological changes and PCR or RT-PCR detection results.Swine Pasteurella was also identified using the bacteria cultivation,Gram stain,Giemsa stain,and biochemical test.Then the variations were analyzed based on ORF2 gene of PCV-2 and Nsp2 partial gene of PRRSV.The results showed that the nucleotide identity between the isolated PCV-2 strains is 94.2%,and the nucleotide identity between the isolated PRRSV strains is 99.7%.Furthermore,the nucleotide identity between the isolated PRRSV strains and the latest reported viruses is 99%,but from 95.8% to 97.2% with viruses reported in 2006.In conclusion,co-infection of PCV-2 and high-pathogenic PRRSV occurred in this large-scale piggery,and the infected PCV-2 strains were derived from different origins while the infected high-pathogenic PRRSVs were highly homologous but the nucleotide variations occurred when compared to virus strains that reported in 2006.

关 键 词：PRRSV NSP2基因 PCV-2 ORF2 混合感染 变异 

分 类 号：S852.659.1[农业科学—基础兽医学] S852.659.2[农业科学—兽医学]