河北香菊中黄酮类成分体外抗氧化活性研究及构效关系探讨  

Study on in vitro antioxidant activity of flavonoids contained in Hebei balmy chrysanthemum and structure-activity relationship

作　　者：孙静[1,2] 黄芸[2] 孙桂波[1] 孙晓波[1] 秦蒙[1] 赵丁[2] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]河北医科大学药学院,河北石家庄050017

出　　处：《中国中药杂志》2012年第13期1958-1962,共5页China Journal of Chinese Materia Medica

基　　金：国家“重大新药创制”科技重大专项(2012ZX09501-001);国家科技支撑计划项目(2008BAI51B00);国家重点基础研究发展计划(973)项目(2009CB522805);河北省科技厅创新药物重大专项(06276408D)

摘　　要：目的:通过研究河北香菊中5种黄酮类成分木犀草素、芹菜素、金合欢素、木犀草素-7-O-β-D-葡萄糖苷、金合欢素-7-O-β-D-葡萄糖苷的体外抗氧化活性,探讨河北香菊的抗氧化机制及黄酮类化合物抗氧化活性的构效关系。方法:采用紫外-可见分光光度法测定对DPPH自由基的清除作用及对H2O2诱导的大鼠红细胞溶血的抑制作用;用硫代巴比妥酸法(TBA)测定对H2O2诱导的大鼠脑匀浆脂质过氧化反应的抑制作用;用谷胱甘肽过氧化物酶(GSH-Px)试剂盒法测定对大鼠血浆中谷胱甘肽过氧化物酶的影响。结果:河北香菊中5种黄酮类成分在清除DPPH自由基、抗红细胞溶血、抑制脑匀浆脂质过氧化、提高谷胱甘肽过氧化物酶活力方面均呈现出较好的活性,活性顺序表现为木犀草素>木犀草苷>芹菜素>金合欢素>金合欢苷。结论:河北香菊所具有的抗氧化活性与其所含黄酮类成分清除自由基、抑制脂质过氧化、提高抗氧化物酶活性有关,且黄酮类化合物的抗氧化活性与其结构中的羟基数目和位置以及糖苷的空间位阻有关。Objective ： To study the antioxidant activity in vitro of five flavonoids contained Hebei balmy chrysanthemum, lute- olin, apigenin, acacetin, acacetin-7-O-β-D-glucoside and acaeetin-7-O-β-D-glucoside and discuss the antioxidant mechanism of Hebei balmy chrysanthemum as well as the structure-activity relationship of antioxidant activity of flavonoids. Method： UV-visible spectro- photometric method was used to determine the DPPH scavenging rate and anti-hemolysis activity of the five flavonoids. The inhibitions on lipid peroxidation in rat brain homogenate were evaluated by measuring the content of MDA, and detected by the TBA method. The effect on glutathione peroxidase （GSH-Px） in rat plasma was detected by GSH-Px kit. Result： The tlavonoids from Hebei balmy chrys- anthemam showed better activity in scavenging DPPH radical, protecting RBC from hemolysis, inhibiting lipid peroxidation in rat brain homogenate, and increasing the activity of GSH-Px in rat plasma. The order of antioxidant efficacy was as follows ： luteolin 〉 luteolin-7-O-β-D-glucoside 〉 apigenin 〉 acacetin 〉 acacetin-7-O-β-D-glucoside. Conclusion： The antioxidant activity of Hebei balmy chrysanthe- mum is related to the effect of flavonoids in scavenging radical, inhibiting lipid peroxidation and increasing the activity of GSH-Px. And the antioxidant activity of flavonoids is related to the number and position of hydroxide radicals and the steric hindrance of glucoside.

关 键 词：河北香菊 黄酮类成分 DPPH 红细胞溶血 脂质过氧化 谷胱甘肽过氧化物酶 构效关系 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]