外翻肠囊法发现元胡止痛片吸收成分群的研究  

Study on absorption ingredients of Yuanhuzhitong pill by everted intestinal sac method

作　　者：陈晓萌[1,2] 张迎春[2] 林朔[3] 杨洪军[2] 吴宏伟[2] 李韶菁[2] 李德凤[2] 许海玉[2] 

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031 [2]中国中医科学院中药研究所,北京100700 [3]临沂大学实验中心,山东临沂276000

出　　处：《中国中药杂志》2012年第13期2005-2011,共7页China Journal of Chinese Materia Medica

基　　金：国家科技支撑计划项目(2008BAI51B03);中国中医科学院基本科研业务费自主选题课题(Z02063);中药质量控制技术国家工程实验室课题(655-30)

摘　　要：目的:建立发现元胡止痛片吸收成分群的外翻肠囊模型法,研究元胡止痛片主要化学成分的吸收特性。方法:采用大鼠外翻肠囊模型,收集3个浓度元胡止痛片饮片提取物给药后不同时间的肠囊液样品,用HPLC进行检测分析,并计算其有效成分的累计吸收量,同时比较元胡止痛片样品与吸收成分间比例。结果:元胡止痛片中8个成分可进入肠囊,分别为原阿片碱、盐酸巴马汀、盐酸黄连碱、欧前胡素、盐酸小檗碱、白当归素、α-别隐品碱、延胡索乙素,此8个成分在空肠、回肠中的吸收速率常数(Ka)随着元胡止痛片提取物浓度增加而增加(P<0.05),符合被动吸收。结论:肠囊对药物成分吸收有选择性,选择外翻肠囊法可评价元胡止痛片多成分的肠吸收特性。Objective： To establish an everted intestinal sac method for determining absorption ingredients of Yuanhuzhitong pill and study absorption characteristics of major chemical constituents of Yuanhuzhitong pill. Method： The everted intestinal sac mod- el was adopted. Intestinal sac fluid samples at different time points after administration of three concentrations of Yuanhuzhitong pill were collected and determined by HPLC. The accumulative absorbed doses of active constituents were calculated, while the proportion between samples of Yuanhuzhitong pill and absorption ingredients was compared. Result： Eight ingredients of Yuanhuzhitong pill can be detected in intestinal sac, they are protopine, palmatine, coptisine, imperatorin, berberine, byakangelicin, α-allocryptopine and tetrahydropalmatine. The absorption rate constants （Ka） of eight constituents increased in jejunum and ileum with the increase in con- centration of Yuanhuzhitong pill extracts （ P 〈 0. 05 ） , indicating a passive absorption. Conclusion： As ingredients are selectively ab- sorbed in intestinal sac, the everted intestinal sac method is selected to assess the intestinal absorption characteristics of ingredients of Yuanhuzhitong prescription.

关 键 词：元胡止痛片 肠外翻技术 HPLC 吸收成分 

分 类 号：R286[医药卫生—中药学]