多途径综合分析比较芍药苷及芍药提取物的吸收差异  

Multi-channel analysis and comparison on absorption difference between paeoniflorin and Paeonia lactiflora extracts

作　　者：陈彦[1] 周蕾[2] 辛然[2] 王晋艳[2] 贾晓斌[1] 

机构地区：[1]江苏省中医药研究院中药新型给药系统重点实验室,国家中医药管理局中药口服制剂释药系统重点研究室,江苏南京210028 [2]江苏大学药学院,江苏镇江212013

出　　处：《中国中药杂志》2012年第13期2012-2016,共5页China Journal of Chinese Materia Medica

基　　金：国家中医药管理局中医药科学技术研究专项(06-07ZP18);江苏省中医药领军人才项目(LJ200913)

摘　　要：目的:多途径比较单体芍药苷与提取物中芍药苷在大鼠肠道的吸收差异。方法:采用大鼠肠灌流模型同时收集肠灌流液、胆汁及血浆样品,结合肠道酶共孵育试验及表观油水分配系数的测定,多途径综合分析比较单体芍药苷与提取物中芍药苷在大鼠肠道的吸收差异。结果:相同浓度下,不同肠段芍药提取物中芍药苷在大鼠肠道的有效渗透系数Peff*均高于单体芍药苷,尤其是在空肠及回肠肠段(P<0.05),但单体芍药苷及提取物中芍药苷在4个肠段的Peff*均较小(Peff*<0.5),单体芍药苷的Peff*在0.209～0.290,提取物中芍药苷的Peff*在0.252～0.333;提取物及单体的胆汁样品、血浆样品及肠道酶共孵育样品中均未检测到芍药苷及其代谢产物。结论:和单体芍药苷相比提取物中芍药苷在大鼠肠道的吸收有明显提高,表明提取物中可能存在其他促吸收成分,但由于芍药苷总体吸收较差,这种影响在短时间(1～3 h)内并未能提高吸收入血及胆汁中芍药苷的含量。Objective： To compare the difference of the absorption difference between paeoniflorin monomer and Paeonia lacti- flora extracts in rat intestinal canals by multi-channels. Method： The rat intestinal perfusion model was established. The intestinal perfusate, bile and plasma samples were collected, in combination of the intestinal enzymes incubation test and the partition coefficient determination, to conduct a nmhi-channel analysis and comparison on absorption difference between paeoniflorin and P. lactiflora ex- tracts. Result ： In the same concentration, permeability coefficient P ff of paeoniflorin in the different intestinal segments of P. lactiflora extract higher than the monomer of paeoniflorin, especially in the jejunum and ileum intestinal segments （ P 〈 0. 05 ）. However, both paeoniflorin monomer and P lactiflora extracts showed less Peff· in four intestinal segments, with the former ranging between 0. 209- 0. 290 and the latter 0. 252-0. 333. No paeoniflorin and its metabolin was determined in bile samples, plasma samples and intestinal enzymes incubation samples of paeoniflorin monomer and P. lactiflora extracts. Conclusion ： Compared with the paeoniflorin monomer, P. lactiflora extract showed significantly increase in Peff·, which indicated that other ingredients in the extract can improve the absorp- tion of paeoniflorin. However, due to the poor absorption of paeoniflorin, this effect fails to increase the concentration of paeoniflorin in bile and plasma within short period of time.

关 键 词：芍药苷 芍药提取物 吸收 大鼠肠灌流模型 多途径分析 

分 类 号：R285[医药卫生—中药学]