一个控制水稻株高QTL qPH3的遗传分析  

Genetic Analysis of a QTL qPH3 for Plant Height in Rice

作　　者：罗炬[1] 邵高能[1] 魏祥进[1] 陈明亮[1] 唐绍清[1] 焦桂爱[1] 谢黎虹[1] 胡培松[1] 

机构地区：[1]中国水稻研究所国家水稻改良中心/水稻生物学国家重点实验室,浙江杭州310006

出　　处：《中国水稻科学》2012年第4期417-422,共6页Chinese Journal of Rice Science

基　　金：国家973计划资助项目(2009AA101101);国家自然科学基金资助项目(31000702);浙江公益技术应用研究资助项目(2010C32G3010027);国家转基因生物新品种培育科技重大专项(2011ZX08001-006)

摘　　要：利用D50/HB277衍生的重组自交系群体,对水稻株高进行QTL分析。在杭州和海南两种环境条件下,共检测到6个控制株高的加性效应QTL,分别位于第1、2、3、4、6和8染色体上,其中,位于第3染色体的QTL qPH3效应较大,在两种环境条件均能稳定表达且贡献率分别为16.52%和16.60%。进一步筛选获得1个剩余杂合体,对其衍生的RHL-F2群体进行遗传分析,其qPH3检测贡献率达76.13%。通过后代验证,最终将其锁定于标记RM16211和RM16237之间物理距离约204kb区域内。这将为该基因的精细定位及克隆奠定基础。A recombinant inbred population derived from the cross between a javonica（D50）and an indica（HB277）rice was used to investigate the genetic factors affecting plant height.A total of six quantitative trait loci（QTLs）with significant additive effect were identified for the plant height,and they were located on chromosome 1,2,3,4,6 and 8.One QTL qPH3 can stably express and have significant additive effects in Hangzhou,Zhejiang Province with the explained variance of 16.52% and Linshui,Hainan Province with the explained variance of 16.60%.One residual heterozygous line（RHL） was selected from the RILs and a RHL-F2 population was constructed.The major QTL qPH3 with the explained variance of 76.13% was detected with the RHL-F2 population.By progeny tests,qPH3 was finally mapped within about 204 kb in the region RM16211-RM16237.These results will be helpful for fine mapping and map-based cloning of this QTL.

关 键 词：水稻 重组自交系 株高 数量性状座位 剩余杂合体 

分 类 号：S511[农业科学—作物学]