逍遥散不同提取部位对慢性轻度不可预知应激模型大鼠行为学及海马中单胺类神经递质的影响  

Effects of Different Extract Fractions of Xiaoyao San on Behavior and Monoamine Transmitters in Hippocampus of Chronic Unpredictable Mild Stress Rats

作　　者：崔杰[1,2] 郭秉荣[1,2] 任艳玲[1,2] 秦雪梅[1] 高晓霞[1] 

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学化学化工学院,山西太原030006

出　　处：《中国医药工业杂志》2012年第7期584-587,共4页Chinese Journal of Pharmaceuticals

基　　金：国家自然科学基金项目(81001688和31070295);山西省基础研究青年科技研究基金(2011021007-1);山西省高等学校中青年拔尖创新人才支持计划资助

摘　　要：建立了慢性轻度不可预知应激(CUMS)模型大鼠,分别灌胃给予逍遥散石油醚部位(1)、95%乙醇部位(2)、1+2及盐酸文拉法辛(阳性药),考察大鼠行为学和海马中单胺类神经递质(去甲肾上腺素、5-羟色胺和5-羟基吲哚乙酸)的变化。采用旷场试验和糖水消耗试验结果及大鼠体重评价其行为学改变,并以HPLC-电化学方法检测海马内单胺类神经递质的含量。结果表明,单用1及1+2联用与阳性药具有相似的抗抑郁效果,对大鼠行为学和3种神经递质的回调均有显著改善;而单用2的疗效不显著,在3种神经递质中仅对去甲肾上腺素有显著的回调作用。The rat model of chronic unpredictable mild stress was established and the effects of different extract fractions of Xiaoyao San were studied The behavior changes and concentrations of monoamino transmitters （including noradrenaline, 5-hydroxytryptamine and 5-hydroxyindoleacetic acid） in hippocampus of the model rats were investigated after ig administration of the petroleum ether fraction （1）, 95 % ethanol fraction （2）, 1＋2 and venlafaxine hydrochloride, respectively. The behavior changes were evaluated with the results of open-field test and sucrose consumption test as well as the rat weight and the concentrations of three monoamino transmitters were determined by HPLC-ECD. The results showed that the groups of 1 alone and 1 combined with 2 showed the antidepressant effects that were similar to venlafaxine hydroxychloride and three transmitters of the above three group rats were significantly improved. While the effect of 2 alone was not significant, 2 only had a significant up-regulation effect on noradrenaline among the three transmitters.

关 键 词：慢性轻度不可预知应激 抑郁症 逍遥散 行为学改变 单胺类神经递质 

分 类 号：R965[医药卫生—药理学]