rhIL-1ra蛋白质的HPLC法测定  

Determination of Recombinant Human Interleukin-1 Receptor Antagonist by HPLC

作　　者：毕引歌[1] 张如亮[2] 贾世香 屠平 韩伟[1] 

机构地区：[1]上海交通大学药学院,上海200240 [2]交晨生物医药技术(上海)有限公司,上海201203

出　　处：《中国医药工业杂志》2012年第7期592-594,共3页Chinese Journal of Pharmaceuticals

摘　　要：建立了分子排阻色谱-高效液相色谱法测定重组人白介素-1受体拮抗剂(rhIL-1ra)蛋白。使用凝胶色谱柱,流动相为0.01 mol/L枸橼酸缓冲液-0.1 mol/L氯化钠溶液,检测波长280 nm。rhIL-1ra蛋白在0.018～2.4 mg/mm浓度范围内线性关系良好。回收率为99.1%,RSD为1.09%。A size exclusion chromatography （SEC）-HPLC method was established for the determination of recombinant human interleukin-1 receptor antagonist （rhIL-lra）. A gel SEC column was used with the mobile phase of 0.01 mol/L sodium citrate buffer and 0.1 mol/L sodium chloride solution at the detection wavelength of 280 nm. The calibration curve of rhIL-1 ra was linear in the range of 0.018 - 2.4 mg/ml. The recovery was 99.1%, with RSD of 1.09 %.

关 键 词：白介素 1受体拮抗剂 高效液相色谱 测定 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.72[化学工程—制药化工]