铁皮石斛快繁技术体系研究  

Rapid propagation technique system of Dendrobium officinale

作　　者：常美花[1] 金亚征[1] 王莉[2] 

机构地区：[1]河北北方学院园艺系,河北张家口075000 [2]唐山师范学院滦州分校,河北唐山063700

出　　处：《中草药》2012年第7期1412-1417,共6页Chinese Traditional and Herbal Drugs

基　　金：河北省科技攻关计划项目(052201124)

摘　　要：目的以铁皮石斛Dendrobium officinale带芽茎段为材料,对铁皮石斛的组织培养进行系统研究,以期建立铁皮石斛的快繁技术体系。方法采用植物组织培养的方法对铁皮石斛外植体的消毒方法、原球茎的诱导、增殖、分化、幼苗生根及瓶苗移栽等进行研究。结果铁皮石斛外植体最佳的消毒方式为75%乙醇消毒30 s,再用0.1%HgCl2消毒10 min;铁皮石斛带芽茎段原球茎诱导的最佳培养基为MS+1.0 mg/L 6-BA+0.5 mg/L NAA+2 g/LAC,诱导率达到34.98%;原球茎增殖的最佳培养基为MS+0.5 mg/L 6-BA+0.8 mg/L 2,4-D+2 g/LAC,增殖率达到89.6%;原球茎分化的最佳培养基为MS+1.2mg/L 6-BA+0.2 mg/L IBA+2 g/L AC,分化率达到89.6%;幼苗生根的最佳培养基配方为1/2 MS+2.5 mg/L NAA+15%土豆汁+2 g/LAC,生根率达到90.4%～92.8%;瓶苗移栽最佳方式为大苗、树皮块苔藓草做基质、基质中添加0.03 mg/L赤霉素,并接种适量菌根真菌Epulorhiza sp.。结论建立了铁皮石斛的快繁技术体系,为铁皮石斛的工业化生产奠定技术基础。Objective Budding stems of Dendrobium offcinale were used as the test materials to systematically investigate tissue culture and establish a rapid propagation technique system. Methods Disinfectant method of explants, induction, proliferation,differentiation,seedling rooting,and transplanting of protocorm in D.offcinalewereresearchedwithtissue culture.Results The best disinfectant method ofexplants is to disinfect by 75% ethanol for 30 s and then by 0.1% HgC12 for 10 min. The best culture medium ofbudding stems induction was MS ＋ 1.0 mg/L 6-BA ＋ 0.5 mg/L NAA ＋ 2 g/L AC with inductivity of 34.98%. The best culture medium of proliferation was MS ＋ 0.5 mg/L 6-BA ＋ 0.8 mg/L 2, 4-D ＋ 2 g/L AC of which proliferation rate could be up to 89.6%. The best culture medium for protocorm differentiation was MS ＋ 1.2 mg/L 6-BA ＋ 0.2 mg/L IBA ＋ 2 g/L AC with differentiation rate at 89.6%.The best culture medium for seedling rooting was 1/2 MS ＋ 2.5 mg/L NAA ＋ 15% potato juice ＋ 2 g/L AC with rooting rate at 90.4%--92.8%. The best way for seedling transplanting was to adopt substratum of big seedling and bark moss as base with 0.03 mg/L gibberellin and Epulorhiza sp. Conclusion Rapid propagation technique system is established in order to lay technique foundation forindustrial production of D. officinale.

关 键 词：铁皮石斛 快繁技术 幼苗移栽 增殖 分化 

分 类 号：R282.21[医药卫生—中药学]