银离子配位柱层析法分离姜油树脂中的姜烯  

Isolation of Zingiberene from Ginger Oleoresin by Silver Ion Coordination Column Chromatography

作　　者：王艳[1] 杜爱玲[1] 杜爱琴[1] 

机构地区：[1]山东大学化学与化工学院,山东济南250061

出　　处：《精细化工》2012年第7期673-677,共5页Fine Chemicals

基　　金：科技部农业科技成果转化项目(2008GB2C600178)~~

摘　　要：以测定姜烯银离子配合物稳定常数为基础,对比姜烯在硝酸银硅胶及硅胶表面的静态吸附情况以及姜油树脂在上述两种层析介质中的薄层分离情况,采用硝酸银硅胶柱层析法分离了姜油树脂中的姜烯。结果表明,当硅胶作为层析介质时,姜烯静态吸附量较小,当硅胶经硝酸银改性后,增强了姜烯的静态吸附能力,吸附行为近似典型的Langmuir吸附。姜烯能与硝酸银形成π配位化合物,其稳定常数为1.46×10-2 L/mol;倍半萜π配位化合物的稳定性与不饱和化合物的结构及溶剂环境有关,因此,硅胶经硝酸银改性后对不饱和化合物具有选择性吸附的能力,使姜烯可以与其他烯类物质分离,所得样品中姜烯质量分数为97.22%,收率为87.15%。On the basis of the stability constant determination of zingiberene-Ag^＋ coordination compound, zingiberene static adsorption on silver nitrate modified silica gel was compared with that on silica gel, and the thin layer chromatography of ginger oleoresin on argentated silica gel G pre-coated plate was compared with that on silica gel G pre-coated plate. Finally the modified silica gel column chromatography was used to isolate zingiberene from ginger oleoresin. The comparison results indicate that zingiberene static adsorption was weak when silica gel was used as the medium of chromatography, and the adsorption capacity was enhanced after silica gel was modified with silver nitrate, when the adsorption behavior conforms to the Langmuir monomolecular adsorption. Zingiberene-Ag^＋ π coordination compound could be formed with the stability constant being 1.46 × 10^-2 L/mol when silver nitrate was added. The stability of sesquiterpene-Ag ＋ coordination compounds is related to their structure and the solvents;consequently when silica gel was modified with silver nitrate, the selective adsorption of zingiberene was increased, and zingiberene was effectively separated from ginger oleoresin and the mass fraction of the final products was 97.22% with 87.15% recovery.

关 键 词：银离子配位 倍半萜 姜烯 催化与分离提纯技术 

分 类 号：TQ028[化学工程]