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作 者:叶绪芳[1] 任刚[1] 苏飞[1] 李胜[1] 刘航[1] 刘铭[1] 张丽[1] 朱青[1] 王涛[1] 管庆虎[1] 许文波[2]
机构地区:[1]贵州省疾病预防控制中心,贵阳市550004 [2]中国疾病预防控制中心病毒病预防控制所
出 处:《现代预防医学》2012年第13期3329-3333,共5页Modern Preventive Medicine
摘 要:目的总结分析贵州省脊灰病毒学监测工作经验;提供贵州省维持无脊灰状态病毒学监测依据。方法采用病毒分离、鉴定与核苷酸序列分析的方法,对贵州省2000~2009年报告的急性弛缓性麻痹(AFP)病例及接触者、健康人群粪便标本进行病毒学检测和结果分析。结果①2000~2009年全省共报告AFP病例3012例,采集报告AFP病例、AFP接触者和健康人群粪便标本共3207例6609份;②从所有粪便标本中分离到脊灰病毒(PV)275例(株),10年总分离率为6.7%,分离到非脊灰其他肠道病毒(NPEV)484例(株),总分离率为11.8%;③275株PV经国家脊灰实验室鉴定,225株为疫苗相关株(81.8%),44株为疫苗变异株(16.0%),6株为疫苗衍生株(VDPVs)占2.2%。至2009年底该省已连续16年未分离到脊灰野病毒;④2000~2009年该省均以优异成绩顺利通过WHO和国家脊灰实验室每年一次的能力验证考核和每2~3年一次的合格脊灰实验室现场认证。结论贵州省脊灰实验室监测系统运转良好,充分发挥了脊灰病毒学监测的作用;监测系统及时发现和处置了疫苗衍生株脊灰病毒(VDPVs)循环,未发现脊灰野病毒株,该省达到了保持无脊灰状态的目标。OBJECTIVE To summary and analyses the work experience of the polio virological surveillance in Guizhou Province and provide the virological surveillance basis for maintaining polio-free status.METHODS The methods of virus isolation,virus identification and nucleotide sequencing were adopted for the virological examination and analysis was made on the result of polio virus surveillance of AFP cases,AFP contacts and healthy crowd by collecting stool specimens in 2000-2009.RESULTS ① In 2000-2009,a total of 3,012 AFP cases were reported in Guizhou Province,and a total of 6,609 part stool specimens were collected from 3,207 reported AFP cases,AFP contacts and healthy crowd.② 275 polioviruses(PV)strains had been isolated form all stool specimens with a virus isolation rate of 6.7%,and 484 non-polio enteroviruses(NPEV)strains had been isolated form all stool specimens with a virus isolation rate of 11.8%.③ 275 strains PV were identified by the National Poliomyelitis Laboratory.Among them,225 strains were vaccine-associated poliovirus,44 strains were vaccine-varied poliovirus,and 6 strains were vaccine-derived poliovirus(VDPVs)(5 strains were type ⅠVDPVs,1 strains were type Ⅱ VDPVs,and all of them were isolated in 2004).It has been 16 years that we haven,t found wild-type poliovirus by the end of 2009 year.④ In 2000-2009,our province polio laboratory successfully passed the yearly Proficiency Test and every 2-3 years Field Accredited of WHO and National Poliomyelitis Laboratory.CONCLUSION Surveillance system of province polio laboratory in Guizhou is running well,and plays well the role of polio virology surveillance.Surveillance system timely discovery and dispose of the vaccine-derived poliovirus strain(VDPVs)circulation,and do not find wild-type poliovirus.Guizhou province achieves the goal of maintaining Polio-free status.
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