7个VNTR位点用于四川省钩端螺旋体菌株的分型研究  

Analysis of genotype of 7 variable number tandem repeat of leptospira interrogans in Sichuan

作　　者：曾林子[1] 郭宗琪[1] 祁滕[1] 刘莉莉[1] 刘谊[1] 罗隆泽[1] 

机构地区：[1]四川省疾病预防控制中心微生物所细菌科,四川成都610041

出　　处：《现代预防医学》2012年第13期3369-3371,3375,共4页Modern Preventive Medicine

基　　金：中华预防医学会科研资金(20102201);四川省卫生厅科学研究项目(100439)

摘　　要：目的掌握了解四川省钩端螺旋体基因分型情况、基因型地理分布特征、各地区的主要优势菌型,为分子流行病学调查中确定暴发流行、追溯传染源和传播媒介、预防控制疫情提供科学依据。方法采用多位点可变数目串联重复序列分析方法(MLVA)对四川分离保存的185株钩体菌株7个VNTR位点进行检测,并用BioNumerics软件进行基因分型聚类。结果 185株钩体菌株成功扩增出164株,阳性率为为88.6%,分为81个MLVA基因型,其中156株致病性钩端螺旋体地方株成功扩增出144株,阳性率为92.3%,分为65个MLVA基因型,涵盖11个血清群34个血清型。结论四川地区钩端螺旋体菌多态性好,基因型别与地理分布呈相关性,可通过MLVA方法为钩体疫情的分子流行病学调查提供及时和准确的科学依据。OBJECTIVE To understand the genotype,geography distribution and dominant genotype of leptospirosis in Si Chuan province,in order to provide the scientific evidence for determining the outbreak,prevalent trace,infection source and diabetic foot,and to prevent and control the epidemic.METHODS 7 VNTRs（variable numbers tandem repeats）of 185 leptospirosis of SiChuan were detected by multiple locus variable number of tandem repeats analysis（MLVA）,and the results were analyzed by software BioNumerics.RESULTS 164 strains of 185 leptospirosis were successfully amplificated,and the positive rate was 88.6%,which were divided into 81 genotypes.144 of 156 leptospia interrogans of Sichuan were successfully amplificated,and the positive rate was 92.3%,which were divided into 65 genotype,including 11 serogroups and 34 serotypes.CONCLUSION The leptospirosis of Sichuan demonstrates good polymorphism,and genotypes are related to geography distribution.It can provide timely and exactly scientific evidence for molecular epidemiology investigation of leptospira by MLVA method.

关 键 词：7个VNTR位点 钩端螺旋体 分型 

分 类 号：R377.5[医药卫生—病原生物学]