健脾复方对人大肠癌移植瘤裸小鼠COX-2、NF-κB和AP-1表达的影响  

Effect of Jianpi Compound Recipe on COX-2,NF-κB and AP-1 in nude mice with human colorectal cancer grafts

作　　者：刘静[1] 史勤[1] 吕祥[1] 冯彦军[1] 

机构地区：[1]上海市中医医院,上海200041

出　　处：《中华中医药杂志》2012年第7期1932-1934,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：上海市自然科学基金项目(No.08ZR1419300);上海市教委科研创新项目(No.08YZ59)~~

摘　　要：目的:研究健脾复方对人大肠癌移植瘤裸小鼠肿瘤环氧化酶2(COX-2)、核因子κB(NF-κB)和激活物蛋白1(AP-1)表达的影响。方法:将40只近交系(BALB/C)裸小鼠建立人大肠癌原位移植瘤模型,随机分入健脾复方组、塞来昔布组、5-Fu组、模型对照组,另设空白对照组,共分组治疗8周。采用逆转录聚合酶链反应(RT-PCR)方法检测COX-2 mRNA表达,采用免疫组化法检测AP-1和NF-κB的表达。结果:健脾复方组、塞来昔布组和5-Fu组裸小鼠COX-2表达均显著低于模型对照组,且与空白对照组相似(P=0.0019)。健脾复方组、塞来昔布组、5-Fu组和模型对照组AP-1表达高于空白对照组(P=0.0065)。健脾复方组和空白对照组NF-κB表达低于5-Fu组和模型对照组(P<0.05),且健脾复方组与空白对照组间差异未见显著性。结论:健脾复方可以下调COX-2的表达,其机制可能与下调其上游因子NF-κB有关。健脾复方对AP-1表达的影响尚需进一步研究。Objective： To investigate the effect of Jianpi Compound Recipe on COX-2,AP-1,and NF-κB expression in nude mice with human colorectal cancer grafts.Method： Human colorectal cancer orthotopic transplanted tumor model was established in 40 BALB/C nude mice.These nude mice were randomized into Jianpi Compound Recipe group,celecoxib group,5-Fu group,and model control group.A blank control group was also established.All groups received respective treatments for 8 weeks.The expression of COX-2 mRNA was assessed by RT-PCR,the expression of AP-1 and NF-κB were assessed by immunohistochemistry method.Results： COX-2 mRNA expressions were significantly lower in Jianpi Compound Recipe group,5-Fu group,Celecoxib group compared with model control group（P=0.0019）,and were similar with blank control group.Expressions of AP-1 were significant higher in Jianpi Compound Recipe group,5-Fu group,Celecoxib group and model control group compared with blank control group（P=0.0065）.Expressions of NF-κB was significantly lower in Jianpi Compound Recipe group and blank control group compared with 5-Fu group and model control group（P〈0.05）,there was no significant difference between Jianpi Compound Recipe group and blank control group.Conclusion： Jianpi Compound Recipe could down-regulate the expression of COX-2,this may due to the down-regulation of NF-κB.The effect on AP-1 needs further investigation.

关 键 词：大肠癌 健脾复方 环氧化酶-2 核因子ΚB 激活物蛋白-1 

分 类 号：R285.5[医药卫生—中药学] R735.34[医药卫生—中医学]