序贯法研究广西不同产地对叶百部总生物碱对小鼠的止咳作用  

Sequential method study on the antitussive effect of the total alkaloids of Stemona tuberosa L.from different production areas in Guangxi

作　　者：王孝勋[1] 黄茂春[1] 赵旭[1] 夏星[1] 黄骞春 

机构地区：[1]广西中医药大学,南宁530001

出　　处：《中华中医药杂志》2012年第7期1935-1937,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广西自然科学基金创新研究团队项目(No.2011GXNSFF018006);广西教育厅面上科研项目(No.200911MS130);广西研究生教育创新计划项目(No.20100106001008M08)~~

摘　　要：目的:采用序贯法研究广西不同产地对叶百部总生物碱止咳作用,为对叶百部品质评价以及临床用药提供理论依据。方法:采用序贯法先进行预实验确定止咳有效剂量,再取10个广西不同产地对叶百部总生物碱作用于不同组别的小鼠,求得各组的EDT50,依次定量判断其止咳活性。结果:不同组别的小鼠EDT50均较空白组有所延长,广西不同产地对叶百部总生物碱均能延长氨水引咳时间(R值>100%),6.9g生药/kg为止咳有效剂量,平均R值为138.8%。结论:广西各产地对叶百部总生物碱均具有止咳作用,而恭城、凤山、靖西产地效果较好。Objective： This study adopted the sequential method to study the cough relieving effect of the total alkaloids of Stemona tuberosa L.from different production areas in Guangxi,to provid theoretical basis for the quality evaluation and clinical medication for Stemona tuberosa L..Methods： Adopting the sequential method,the effective dosage for relieving cough was determined by preliminary experiment.EDT50 of the mice was figured out after giving mice the total alkaloids from 10 different production areas of Stemona tuberosa L..Results： The results indicated that total alkaloids of Stemona tuberosa L.from different production areas could prolong the time after which ammonia water-induced cough occurred（R100%）;R was 138.82% in average.Conclusion： The total alkaloids of Stemona tuberosa L.from different production areas had the efficacy of relieving cough,but there was significant difference especially in the Gongcheng,Fengshan,Jingxin areas.

关 键 词：对叶百部 总生物碱 止咳作用 不同产地 序贯法 

分 类 号：R284[医药卫生—中药学]