黄栌有效成分漆黄素提取工艺研究  

Fisetin extraction process of cotinus coggygria's active ingredients

作　　者：李建华[1] 段丹丹[1] 马聪玲[1] 申庆亮 

机构地区：[1]武警河南总队医院药剂科,郑州450052 [2]济南军区中药研究中心,新乡453000

出　　处：《中华中医药杂志》2012年第7期1956-1958,共3页China Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:筛选黄栌中有效成分漆黄素最佳提取工艺的研究。方法:采用单因素考察和L9(34)正交试验设计法,以黄栌中漆黄素的含量为考察指标,取4因素(提取溶媒、提取时间、溶媒pH、溶媒量)3水平,优选漆黄素提取工艺。结果:最佳提取工艺为A2B3C3D3,即以65%、25倍药材量的乙醇为提取溶媒,调pH9,每次90min以提取2次为宜。结论:该提取工艺设计合理、可行,工艺稳定,重现性好,提取率高(1.03%),为进一步开发利用黄栌药用资源提供指导。Objective： To select out the optimal extraction process of fisetin of cotinus coggygria.Methods： We adopted a single factor and L9（34） orthogonal experimental design method.The index was the content of fisetin in cotinus coggygria.We took four factors（extraction solvent,extraction time,solvent pH,solvent volume）,three levels,preferred extraction process of fisetin.Results： The optimum extraction was A2B3C3D3 which used 65% and 25 times amount of ethanol at pH9 as extraction solvent,and extracted 2 times,90min each time.Conclusion： The optimized extraction process is stable and feasible,with good reproducibility,high extraction rate（1.03%）,provided a reference for further exploitation of the medicinal resources of the cotinus coggygria.

关 键 词：正交实验 黄栌 漆黄素 提取工艺 

分 类 号：R284[医药卫生—中药学]