采用微卫星DNA标记分析我国2个地方鹅种异地保种效果  

Conservation Efficiency of Local Goose Breeds in Allopatrty Farm as Revealed by Microsatellite Markers

作　　者：张扬[1] 陈阳[1] 荣光辉[1] 段修军 李欣钰[3] 徐琪[1] 陈国宏[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]江苏省畜牧兽医职业技术学院,江苏泰州225300 [3]江西农业大学动物科技学院,江西南昌330045

出　　处：《中国畜牧杂志》2012年第13期1-5,共5页Chinese Journal of Animal Science

基　　金：现代农业产业技术体系建设专项资金(nycytx-45-04)

摘　　要：本研究以10对荧光标记微卫星引物分析了我国2个地方鹅种(狮头鹅和皖西白鹅)原产地与基因库(泰州)共4个群体的保种效果。检测判定了狮头鹅和皖西白鹅共240个个体的基因型,通过计算4个群体的优势等位基因频率(P)i、期望杂合度(He)、多态信息含量(PIC)、群体内近交系数(Fis)分析了狮头鹅和皖西白鹅群体内和群体间的遗传变异,采用配对试验均数差异t检验比较了基因库保种群体与原产地群体的遗传差异,基因库保种群体与原产地群体间的Pi无显著差异(P>0.05);He、PIC均略高于原产地群体,但差异均不显著(P>0.05);Fis低于原产地群体,差异不显著(P>0.05)。结果说明,基因库较好地保存了这2个品种并在一定程度上丰富了品种的遗传多样性,表明对我国地方鹅种采取异地小群保种的方法是可行的。Ten fluorescence labeling microsatellite markers were used to analyze the conservation efficiency of two local goose breeds（Shitou goose and Wanxi goose） containing 4 populations between the gene pool（Taizhou）and the place of origin.The genotypes of 240 individuals were detected and the genetic variation between populations of these two breeds were analysed by computing the advantage allele frequencies,expected heterozygosity,polymorphism information content（PIC）and population inbreeding coefficients（Fis）.The genetic variations between the gene pool populations and the original ones by using paired experiment mean t test.The advantage Pi of these two breeds were not significantly different in the four populations（PST=0.197,PWX=0.219）;He,PIC were slightly higher than the original populations,but the differences were also not significant（PHe=0.063,PPIC=0.144,PHe=0.230,PPIC=0.195）;Fis were lower than the original populations but the differences were not significant（PST=0.111,PWX=0.130）.The results showed that these two breeds were preserved perfectly in the gene pool and the genetic diversity was also improved to a certain extent.It was feasible that local goose breeds were conserved on allopatrty farms.

关 键 词：保种效果 微卫星DNA标记 皖西白鹅 狮头鹅 

分 类 号：S835.2[农业科学—畜牧学]