ELSD-HPLC法测定和丹舒胶囊中黄芪甲苷含量  

Determination of Astraloside in Hedanshu Capsules by ELSD-HPLC

作　　者：张红艳[1] 张淑玲[1] 陆军[2] 夏超[2] 

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]江苏省药物研究所,江苏南京210009

出　　处：《辽宁中医药大学学报》2012年第7期95-96,共2页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：福建省科技计划社会发展重点项目(2011Y0035)

摘　　要：目的：采用高效液相色谱法测定和丹舒胶囊中黄芪甲苷含量。方法：采用DiamonsilTM（4.6mm×250mm,5μm）色谱柱;乙腈-水（36：64）为流动相;流速1.0mL.min-1;漂移管温度：105℃;载气流速：2.7L/min。结果：黄芪甲苷进样量在1.21~12.19μg范围内呈现良好的线性关系,平均加样回收率为99.33%,RSD为1.03%（n=9）。结论：本法简便、准确,可作为该制剂的质量控制方法。Objective ： To determine the content of Astraloside in Hedanshu Capsules by ELSD- HPLC. Methods ： Use the DiamonsilTM （ 4.6mm × 250mm, 5μ m ） column, its mobile phase was acetonitrile-water （ 36 ： 64 ）. The flow rate was 1.0mL/min, drift tube temperature was 105 ℃, and gas flow rate was 2.7L/min. Result：It showed that the Astraloside had a good linearity within the range of 1.21-12.19 μ g （r=-0.9998）.The average recovery was 99.33 %, and RSD=1.03% （n=9）.Conclusion：This method is simple and accurate for determination of Astraloside in Hedanshu Capsules.

关 键 词：和丹舒胶囊 黄芪甲苷 高效液相色谱法-蒸发光散射检测法 含量测定 

分 类 号：R927.2[医药卫生—药学]