乳香酯对ox-LDL诱导的巨噬细胞表达单核细胞趋化因子的影响  

The effect of gum mastic on ox-LDL-induced the MCP-1 production in macrophages

作　　者：郭冬娜[1] 廖平川[1] 王晓卉[1] 

机构地区：[1]山西医科大学第一医院急诊科,太原030001

出　　处：《中华临床医师杂志（电子版）》2012年第13期73-75,共3页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的探讨乳香酯对氧化型低密度脂蛋白(ox-LDL)所致的巨噬细胞单核细胞趋化因子(MCP-1)释放的影响及相关分子机制。方法以ox-LDL、ox-LDL加不同浓度的乳香酯处理人单核细胞THP-1,采用酶联免疫吸附测定细胞上清液中MCP-1的浓度,免疫印迹法检测细胞质中p65/NF-κB和IκBα的表达水平以及细胞核内p65/NF-κB的表达水平。结果乳香酯能够显著减少ox-LDL所致的巨噬细胞MCP-1的表达,且p65/NF-κB的核移位减少,IκBα的降解水平明显降低。结论乳香酯减少ox-LDL所致的巨噬细胞MCP-1的表达与其调节磷酸化IκBα的表达和NF-κB的核-浆转位有关。Objective To explore the effect of gum mastic on the MCP-1 production in macrophages stimulated by oxidized low-density lipoprotein（ox-LDL）. Methods THP-1 macrophage were treated with ox-LDL alone or accompanied with different concentrations of gum mastic, the level of MCP-1 in cell culture supernatants was measured by ELISA,the level of nuclear pool and plasma of p65/NF-KB protein was examined by Western blot for NF-KB nuclear transloeation analysis, and the degradation of IKBα was determined by western blot. Results Gum mastic inhibited the MCP-1 release induced by ox-LDL,and gum mastic also inhibited NF-KB translocation and the IKBα degradation. Conclusions The effect of gum mastic block the production of the MCP-1 is related to inhibiting the NF-KB pathway.

关 键 词：脂蛋白类 LDL 趋化因子CCL2 乳香属 

分 类 号：R285[医药卫生—中药学]