黄芪甲苷对病毒性心肌炎小鼠TLR4、NF-κB及TNF-α表达的影响  

Effect of astragaloside on expression of TLR4,NF-κB and TNF-α in mice with viral myocarditis

作　　者：周欣[1] 刘春梅[1] 胡安娜[1] 杜自强[1] 王利利[1] 武怡[1] 孟令建[1] 路明[1] 

机构地区：[1]徐州医学院附属医院儿科,江苏省221000

出　　处：《中华临床医师杂志（电子版）》2012年第13期235-238,共4页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的探讨黄芪甲苷对病毒性心肌炎小鼠Toll样受体4(TLR4) mRNA、核因子-κB(NF-κB) mRNA表达及血清肿瘤坏死因子(TNF-α)的影响。方法将80只雄性BALB/c小鼠随机分为柯萨奇病毒感染组(感染组,30只)、黄芪甲苷治疗组(治疗组,30只)和正常对照组(对照组,20只)。感染组和治疗组小鼠腹腔注射柯萨奇病毒B3(CVB3),建立病毒性心肌炎模型。治疗组于注射病毒后30min开始灌服黄芪甲苷,每日1次×7d;感染组以生理盐水灌胃;对照组用不含病毒的Eagle液腹腔注射、生理盐水灌胃。各组分别在7d、14d各随机抽取8只小鼠处死,HE染色后检测心肌病理积分、逆转录-聚合酶链反应(RT-PCR)检测心肌中TLR4 mRNA和NF-κB mRNA的表达,ELLSA法检测血清TNF-α的含量。结果感染组心肌病理积分、TLR4 mRNA、NF-κB mRNA表达及血清TNF-α的含量较对照组明显增加(P<0.01),治疗组心肌病理积分、TLR4 mRNA、NF-κB mRNA表达及血清TNF-α的含量较感染组明显降低(P<0.05)。结论黄芪甲苷可以通过调节TLR4、NF-κB及TNF-α在病毒性心肌炎中的表达,减轻心肌损伤。Objective To study the effect of astragaloside on expression of TLR -4, NF-KB and TNF-α in mice with viral myecarditis. Methods Eighty male BALB/c mice were randomly divided into three groups including the Coxsackie-virus B3 （ CVB3 ） infected group （ n = 30 ）, the CVB3 infected and astragaloside-treated group （ n = 30 ） and control group （n = 20）. The first two groups were intraperitoneally inoculated with CVB 3 to induce myocarditis, control group with virus-free Eagle＇s medium instead. Astragaloside orally administered everyday in astragaloside- treated group from 30 minutes ＇after injecting virus for 7 days. Control group and myocarditis group were used normal saline instead. In the end of first and the second week, 8 mice from each group to select randomly were killed to examine the myocardial pathologic score by HE staining , expression of TLR4 mRNA, NF-KB mRNA by RT-PCR and serum TNF-α volume by ELLSA. Results Significant up-regulation of TLR4 mRNA, NF-KB mRNA expression levels ,myocardial pathologic score ,and serum TNF-α volume were observed in mice of the CVB3 infected group in comparison with control group （ P 〈 0. 01 ）. TLR4 mRNA, NF-KB mRNA expression levels, myocardial pathologic score,and serum TNF-α volume were remarkably decreased in astragaloside-treated group compared with CVB3 infected group （P 〈 0. 05 ）. Conclusions Astragaloside may exerts some beneificial effect by regulating the expression of TLR4 ,NF-KB ,TNF-α,which contribute to reduce the myocardial injury.

关 键 词：心肌炎 NF-ΚB 肿瘤坏死因子Α 黄芪甲苷 

分 类 号：R285.5[医药卫生—中药学]