TNF-α、IL-10在变应性鼻炎中的作用研究  

The roles of TNF-α and IL-10 in allergic rhiultis

作　　者：王鑫[1] 张燕[1] 李淼[1] 王彤[2] 焦慧[1] 高志光[1] 张春玉[3] 

机构地区：[1]黑龙江省医院耳鼻咽喉科,150036 [2]黑龙江省医院检验科,150036 [3]哈尔滨医科大学遗传学教研室,150081

出　　处：《国际免疫学杂志》2012年第4期317-319,共3页International Journal of Immunology

基　　金：黑龙江省卫生厅科研项目（2011-676）

摘　　要：目的通过检测变应性鼻炎（AR）患者血清TNF—α．、IL-10在治疗前后的表达变化，探讨二者在变应性鼻炎中的作用。方法收集33例正常健康对照组以及48例变应性鼻炎患者行脱敏治疗前后的血清标本，应用双抗体夹心ELISA方法测定血清中TNF-α、IL-10的表达水平，SPSS12．0软件进行统计学分析。结果TNF．仅在AR患者血清中高表达，与对照组相比差异具有统计学意义（P=0．0028），脱敏治疗后其表达下降，治疗前后相比有统计学差异（P=0．0032）；IL-10在患者血清中表达低于对照组，差异具有统计学意义（P=0．018），脱敏治疗后其表达上升，与治疗前相比差异有统计学意义（P=0．024）；TNF-α、IL-10在AR患者中的表达呈负相关（r=-0．413，P〈0．05）。结论TNF-α和IL-10在变应性鼻炎发病过程中可能起到重要作用。Objective To explore the role of TNF-α, IL-10 in allergic rhinitis（AR） through the detection of their serum levels changes. Methods The serum samples were collected from 33 normal healthy controls and 48 patients with AR before and after the desensitization treatment. The serum levels of TNF-α, IL-10 were detected by double antibody sandwich ELISA. SPSS 12.0 software was used for statistical analysis. Results AR patients showed high level of TNF-α compared with the control group （P = 0.0028 ）. After desensitization treatment,TNF-α was decreased significantly （P 〈 0.01 ） decreased compared with the pre-treatment and there was high significant difference （P = 0.032） ;The level of IL-10 in the serum of patients was with lower than the control group, the difference was significant （P = 0.018 ）. With desensitization therapy, its level was rose compared with the pre-treatment and significant difference was found （P = 0.024）. Besides, the level of TNF-α and IL-10 in AR patients showed a negative correlation （ r = - 0. 413, P 〈 0.05 ）. Conclusion TNIα and IL-10 play an important role in the pathogenesis of allergic rhinitis.

关 键 词：TNF-Α IL-10 变应性鼻炎 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]