鸭副粘病毒弱毒株的选育  

Selection of Attenuated Vaccine Strain of Duck Paramyxovirus

作　　者：程晓霞[1,2] 陈少莺[1,2] 朱小丽[1,2] 林锋强[1,2] 陈仕龙[1,2] 王劭[1,2] 李兆龙[1,2] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福建福州350013 [2]福建省畜禽疫病防治工程技术研究中心,福建福州350013

出　　处：《福建农业学报》2012年第5期457-460,共4页Fujian Journal of Agricultural Sciences

基　　金：福建省财政专项--福建省农业科学院科技创新团队建设项目(STIF-Y02);福建省农业科学院科技计划项目(B2010-02)

摘　　要：应用SPF鸡胚成纤维细胞(CEF)连续传代培育出鸭副粘病毒弱毒疫苗株,并测定该弱毒株的部分生物学特性。结果显示:该弱毒株已失去对无母源抗体番鸭的致病性,在雏番鸭体内连续肓传5代,未见毒力返强现象;对CEF的TCID50稳定在10-6.0～10-6.5.(0.1mL)-1;无母源抗体的番鸭免疫不同代次弱毒后7d攻击鸭副粘病毒强毒,保护率均在90%以上。结果表明该弱毒株安全性好、遗传性稳定、免疫原性强,可用于制备鸭副粘病毒病疫苗。An attenuated vaccine strain of Duck Pararnyxovirus （DPMVa） was selected and obtained by passage serial within SPF chicken embryo fibroblast （CEF）, and some biological property of it were detemined. The DPMVa was non-pathogenic to Muscovy ducks without maternal antibody ; and was non-viulenee through five serial passages in one-day-old Muscovy ducks; TCID50to CEF stable between 10^-6.0 -10^-6.5 . （0. 1 mL） 1. The protection rate of the attenuated strain was above 90% when challenged with a virulent strain of DPMV 7 days after vaccination. The results indicated that attenuated vaccine strain had excellent safety, effective immunogenicity and genetic stability, which could be used as a vaccine against DPMV.

关 键 词：鸭副粘病毒 弱毒株 选育 

分 类 号：S858[农业科学—临床兽医学]