灰梨孢菌产孢培养基筛选  

Screening of sporogenous medium for Pyricularia grisea (Cooke) Sacc

作　　者：霍秀娟[1] 李朝生[2] 韩美丽[1] 陆荣生[1] 

机构地区：[1]广西农业科学院植物保护研究所,南宁530007 [2]广西农业科学院生物技术研究所,南宁530007

出　　处：《南方农业学报》2012年第5期605-608,共4页Journal of Southern Agriculture

基　　金：广西青年科学基金项目(桂科青0832021;桂科青0991017)

摘　　要：【目的】筛选操作方法简便、产孢效果好、适宜于大多数灰梨孢菌的产孢培养基,为灰梨孢菌研究提供一定的技术支持。【方法】从水稻、香蕉和杂草马唐上分离获得6个灰梨孢菌菌株,接种于紫花鸭趾草、高粱粒和大麦粒等9种培养基上,测定不同培养基对灰梨孢菌产孢量的影响,并比较分析紫花鸭趾草培养基对病菌致病力的影响。【结果】高粱粒、大麦粒和紫花鸭趾草培养基对6个菌株孢子形成均有明显的促进作用,以紫花鸭趾草培养基的促进作用最明显。与常用的高粱粒培养基相比,6个菌株在紫花鸭趾草培养基上形成的病菌分生孢子对香蕉和水稻的致病力没有明显差异。在4℃冰箱中,用紫花鸭趾草培养基保存蕉瘟病和稻瘟病菌菌种,两年后病菌仍具有生活力。【结论】紫花鸭趾草培养基是适宜于灰梨孢菌分生孢子形成的理想培养基,紫花鸭趾草培养基也适合于保存灰梨孢菌。[Objective]To provide technical support for Pyrieularia grisea research, common effective sporogenous medium with simple, suitable operation was screened in the present experiment. [ Method ]Six Pyricularia grisea isolates obtained from rice, banana, and crab-grass were inoculated on nine kinds of media, e.g., C. communis, sorghum grain and barleycorn, etc. Effects of different media on sporulation quantity of Pydcularia grisea were investigated, and pathogenicity of Pyrieularia grisea as assayed in C. eonmmnis media. [Result]Sorghum grain, barleycorn and C. communis media pronouncedly stimulated the sporulation in six strains with C. communis bested. Compared to commonly-used sorghmn grain nledium, spores cultured in C. communls media had similar pathogenicity towards rice and banana. Magnaporthe oryzae and banana blast strains in C. communis medium could he preserved at 4℃ for two years. [Conelusion]C. communis media was found tobe the most suitable media for spores of Pyricularia grisea, and it was also found to conserve Pyrieularia grisea.

关 键 词：灰梨孢菌 稻瘟菌 蕉瘟菌 培养基 产孢量 

分 类 号：S432.44[农业科学—植物病理学]