样品采集和储存所用耗材对微量元素定量分析的影响  

Impact of specimen collection and storage consumable products on trace element quantitative analysis

作　　者：宋艳双[1] 顾永恩[1] 巴特[1] 翟敏[2] 濮吉[1] 沈臻霖[3] 唐仕川[3] 贾光[1] 

机构地区：[1]北京大学公共卫生学院劳动卫生与环境卫生学系,100191 [2]济宁医学院公共卫生学院社会医学系 [3]北京市劳动保护科学研究所职业安全健康重点实验室

出　　处：《中华预防医学杂志》2012年第7期640-643,共4页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金（81072281）;卫生行业科研专项（200902006）;北京市科学技术研究院科技创新工程（PXM2011-178304-112365）

摘　　要：目的探讨样品采集、储存所用耗材对微量元素定量分析的影响。方法市售A、B、C、D4种品牌尿液采集和储存耗材（每种2个样品），经体积分数为1％的HNO，浸泡2～4h；O、P、Q品牌采血装置（每种3个样品）采集超纯水，模拟采血过程；X、Y、Z品牌粉尘采样滤膜（每种2个样品），以浓硝酸冷消化12h后进行微波消解。对上述采集、储存过程中所得溶液以电感耦合等离子体质谱仪测定镉、钴、铬、铜、铁、锰、钼、镍、铅、硒、锡、钛、钒、锌等14种元素的浓度。结果尿液采集和储存耗材的元素本底值以均数表示，B品牌5ml冻存管所检测14种元素本底值均较低，其含量范围为0．001～0．350ng/ml。A品牌50ml离心管的铜元素、c品牌5ml冻存管的铬元素、D品牌1．5ml离心管的锌元素本底值较高，分别为1．900、1．095和1．368ng/ml。血液采集装置元素本底值以x±s表示，0、P、Q品牌铬元素的本底值分别为（0．120±0．017）、（0．337±0．093）和（0．360±0．035）ng/ml，铜元素的本底值分别为（0．050±0．001）、（0．017±0．012）和（0．103±0．015）ng／ml，铅元素的本底值分别为（0．057±0．072）、（0．183±0．118）和（0．347±0．006）ng／ml，钛元素的本底值分别为（7．883±0．145）、（8．863±0．190）和（8．613±0．274）ng／ml，锌元素的本底值分别为（2．240±0．573）、（42．140±22．756）和（8．850±3．670）ng/ml。3种品牌采血装置铬、铜、铅、钛、锌元素的本底值差异均有统计学意义（P值均〈0．05）。空气采样滤膜元素本底值以均数表示，x品牌滤膜铬、镍元素的本底值较低，分别为17．000、15．400ng/张，其他元素的本底值较高，镉、钴、铜、铁、锰、钼、铅、硒、锡、钛、钒、锌本底值分别为0．250、0．550、48．500、690．000、25．500、0．900、6．500、10．550、7．950、10．500、0Objective This study aimed to explore the impact of specimen collection and storage consumable products on trace element quantitative analysis. Methods Devices and consumable products of different brands used in specimen collection or storage were selected and treated separately as below：urine collection and storage tubes （Brand A, B, C and D, 2 samples for each brand） were treated with 1% of HNO3 volume fraction for 2 - 4 h ; blood taking device （ Brand O, P and Q, 3 samples for each brand） were used for uhra-pure water samples collecting as simulation of blood sampling ; dust sampling filters （ Brand X, Y and Z, 2 samples for each brand ） were cold digested by nitric acid for 12 h, followed by microwave digestion. Then cadmium, cobalt, chromium, copper, iron, manganese, molybdenum, nickel, lead, selenium, stannum, titanium, vanadium and zinc concentrations in the solutions obtained during the course of collect or storage were quantified by inductively coupled plasma mass spectrometer. Results For the urine collection and storage consumable products, background values of elements were discribed as mean of parellel samples. The consentration of 14 quantified elements were relatively low for 5 ml cryogenic vials （ brand B） with background values range of 0. 001 -0. 350 ng/ml. The background values of copper of 50 ml centrifuge tubes（ brand A） ,chromium of 5 ml cryogenic vials（ brand C） and zinc of 1.5 ml centrifuge tubes （brand D） were relatively high,which were 1. 900,1. 095 and 1. 368 ug/ml,respectively. Background values of elements in blood sampling devices were discribed as x ±s. Background values of chromium for brand O, P and Q were （0. 120 ±0. 017 ）, （0. 337± 0. 093 ） and （0. 360 ± 0. 035 ） ng/ml ; for copper were （0. 050± 0. 001 ）, （0. 017 ± 0. 012） and （0. 103 ± 0. 015 ）ng/ml; for lead were （0. 057 ±0. 072）, （0. 183 ± 0. 118 ） and （0.347 ±0.006） ng/ml;for titanium were （7.883 ± 0.145）, （8.863 ± 0.190） and （8.613 ± 0.

关 键 词：质量控制 痕量元素 样品采集 

分 类 号：R134[医药卫生—劳动卫生]