戴云山羊多胎性能候选基因BMPR-IB、BMP15、GDF9的应用研究  

Applied Research about BMPR-IB,BMP15 and GDF9 as Candidate Genes for Fecundity in Daiyun Goat

作　　者：刘远[1] 李文杨[1] 林仕欣[1] 张晓佩[1] 高承芳[1] 林碧芬[1] 董晓宁[1] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福州350013

出　　处：《中国农学通报》2012年第17期23-27,共5页Chinese Agricultural Science Bulletin

基　　金：福建省省属公益类科研院所基本科研专项项目"戴云山羊高繁系的选育及关键配套技术研究"(2011R1025-9);福建省农业科学院青年人才创新基金项目"戴云山羊生长发育曲线拟合分析及应用"(2010QC-14)

摘　　要：为初步探索戴云山羊多胎性能的分子机制。以控制部分绵羊品种高繁殖力的BMPR-IB、BMP15和GDF9基因作为候选基因,采用PCR-RFLP方法分析戴云山羊BMPR-IB、BMP15、GDF9基因多态性与繁殖性状的关系。结果表明:多胎品种戴云山羊及南江黄羊在BMPR-IB基因的相应位置上并未发生与BooroolaMerino羊相同的突变,同时也未检测到BMP15的FecXI、FecXH、FecXB基因、GDF9的FecGH基因,因此排除了这5个突变位点影响戴云山羊高繁殖力性状的可能性。由于戴云山羊的BMPR-IB、BMP15、GDF9的全基因序列信息的缺乏,尚不能完全断定3个基因对戴云山羊高繁殖力性状没有影响。To study the Molecular Mechanisms of fecundity trait in Daiyun Goat. BMPR-IB, BMP15 and GDF9 which control the fecundity of sheep were studied as candidate genes. PCR-RFLP was applied to analyze the relationship between the gene and genotype polymorphisms of BMPR-IB, BMP15 and GDF9 in Daiyun Goat and their prolific trait. The results showed that there was no same mutation in BMPR-IB gene of Daiyun Goat as that of Booroola Merino. The Feci, FecXh, FecXb of BMP15 and FecGH of GDF9 were not found. Then it was ruled out the possibility that the fecundity of Daiyun Goat were affected by the mutations of BMPR-IB, BMP15 and GDF9 gene. As we didn＇ t knew the whole gene sequence of the three genes in Daiyun Goat, it＇ s hard to determine whether these genes affected the fecundity of Daiyun Goat.

关 键 词：BMPR-IB BMP15 GDF9 戴云山羊 繁殖性状 

分 类 号：S826.92[农业科学—畜牧学]