兴奋或抑制GTH细胞内PKC对FSH分泌的影响  

The Influence of Excited or Inhibited PKC in GTH Cells on the Secretion of FSH

作　　者：王新[1,2] 谭建华[3] 赖小平[2] 

机构地区：[1]青岛农业大学动物科学技术学院,山东青岛266109 [2]广州中医药大学中药学院,广州510006 [3]解放军军事医学科学院十一研究所,长春130062

出　　处：《中国农学通报》2012年第17期72-74,共3页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"不同脉冲频率GnRH调节大鼠GTH表达的信号转导机制研究"(30571356);广东省科技计划项目"治疗心血管及代谢性疾病创新中药国际合作平台的建设"(2011B050300021);青岛农业大学高层次人才启动基金"ICA作用GTH细胞的受体后信号转导机制研究"(630810);青岛农业大学实验技术课题"小鼠茜素红;Alcian蓝重染色骨组织标本制作方法的改进"(SYJK09-05);青岛农业大学教学研究课题"<中兽医学>教学方法的研究"(XJG0906);广州中医药大学博士后科研启动金"不同脉冲频率GnRH调节大鼠GTH表达的信号转导机制研究"(B3YH1008)

摘　　要：为了分析大鼠FSH分泌受体后信号转导机制。将GTH细胞用PMA或H7处理后,用GnRH脉冲刺激,再用ELISA法检测其FSH分泌量,并与空白对照组比较。结果表明:PMA能显著提高GTH细胞中PKC活性,H7能显著降低GTH细胞中PKC活性,PMA或H7都不会影响GTH细胞的cAMP含量,GTH细胞PKC活性显著影响FSH的分泌,FSH随着PKC活性的升高而升高,随着cAMP的降低而降低。得出PKC-Ca2+是GnRH脉冲刺激所引起的FSH分泌的受体后的信号转导途径。In order to analyze the post-receptor signal transduction mechanism of the secretion of FSH in rats. The GTH cells was stimulated by GnRH after the activity of PKC in them was treated by PMA or H7. FSH was detected by ELISA, which was compared with the control group. The results showed that PMA could significantly improved the activity of PKC in GTH cells, which H7 could significantly reduced. Neither PMA nor H7 could not influenced the content of cAMP significantly in GTH ceils. The activity of PKC in GTH ceils influenced the secretion of FSH significantly, that was to say, the level of FSH rose with the rise of PMA and fallen with the falling of H7. The conclusion was drawn that PKC-Ca2 ＋ was the post-receptor signal transduction pathway that the ceils stimulated by GnRH impulse secreted FSH.

关 键 词：促性腺激素释放激素 促卵泡激素 信号转导 蛋白激酶C 

分 类 号：S852.21[农业科学—基础兽医学]