极端高温对木薯单爪螨保护酶活性的影响研究  

Effect of Extreme High Temperature on the Enzymes of Cassava Green Mite Mononychellus tanajioa(Acari:Tetranychidae)

作　　者：卢芙萍[1,2] 符悦冠[1,2] 郭容琦[3] 蔡源[2] 经福林[1,2] 徐雪莲[2] 卢辉[2] 陈青[2] 

机构地区：[1]海南大学环境与植物保护学院,海口570228 [2]中国热带农业科学院环境与植物保护研究所/农业部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物监测与控制重点实验室/海南省热带作物病虫害生物防治工程技术研究中心,海南儋州571737 [3]云南省农业科学院热带亚热带经济作物研究所,云南保山678000

出　　处：《中国农学通报》2012年第18期223-230,共8页Chinese Agricultural Science Bulletin

基　　金：现代农业产业技术体系建设专项资金"木薯产业技术体系害虫防控岗位科学家项目"(CARS-12-hncq);中央级公益性科研院所基本科研业务费专项"重要危险性害螨无害化防控资源的收集与评价"(2011hzs1J014);公益性行业(农业)科研专项资助"入境台湾果蔬危险性有害生物防控新技术研究与示范"(200903034-5);海南省重点科技计划项目"热带作物地下害虫无害化综合防控关键技术研究与示范"(ZDXM20110032);海南大学‘211工程’建设项目"海南大学环境与植物保护学院研究生创新平台"

摘　　要：为探讨木薯单爪螨的生态适应性机理,揭示其种群扩散蔓延机制。开展了极端高温胁迫对木薯单爪螨各龄螨的存活率及几种保护酶(多酚氧化酶(PPO)、过氧化物酶(POD)、抗坏血酸过氧化物(AsA-POD)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD))活性的影响研究。结果表明:经42℃高温处理,木薯单爪螨各龄螨最多仅可存活66h,不能进一步发育。PPO和CAT活性在幼螨体内显著下降,而在前若螨到成螨却显著升高,而尤以前若螨升高最高;与对照相比,AsA-POD活性在幼螨体内无变化,但在前若螨体内可升高达对照的3.286倍,到后若螨期升高至对照的1.923倍,成螨期无显著变化;各龄螨体内POD和SOD的活性均无显著变化。因此,说明PPO、CAT和AsA-POD活性变化可能在木薯单爪螨抵抗高温过程中具有重要作用。Temperature was one of the important ecological factors for development and reproduction of Mononychellus tanajioa.In order to learn about the ecological adaptation of M.tanajoa after its invasion into our country and provide a foundation for the monitoring early warning and effective prevention as well as analyze its invasion,diffusion and ecological adaptation mechanism,this study researched the effect of extremely high-temperature（42℃） treatment on the survive and the activity changes of several protective enzymes [polyphenol oxidase（PPO）,peroxidase（POD）,ascorbate oxidase（AsA-POD）,catalase（CAT） and superoxide dismutase（SOD）] by spectrophotometric endpoint assay after the mites in each growth period were treated for different lengths of time at 42℃.The results showed that 42℃ had important effect on the protective enzymes of M.tanajioa.Activities of PPO and CAT of larvae significantly decreased under 42℃ treatment,but that of them were increased in protonymphs and adults,especially in protonymphs.In 42℃,AsA-POD activities of larvae and adults had no changes,but that increased 3.286-fold in protonymphs and 1.923-fold in deutonymph relative to that in 25℃.High temperature of 42℃ had no effect on POD and SOD activities of M.tanajioa.These results suggested that the tolerance of M.tanajioa to high temperature could be associated with PPO,CAT and AsA-POD,but not with POD and SOD activities.

关 键 词：木木薯单爪螨 高温 保护酶 活性 生态适应性 

分 类 号：S433.2[农业科学—农业昆虫与害虫防治]