雌激素对前成骨细胞胰岛素受体底物-1的影响  

EFFECT OF ESTRADIOL ON THE EXPRESSION PROFILE OF IRS-1 DURING MC3T3-E1 CELLS DIFFERENTIATION

作　　者：何玉玲[1] 周后德[2] 隋国良[2] 谢辉[2] 廖二元[2] 

机构地区：[1]广西医科大学第一附属医院代谢糖尿病中心,南宁530021 [2]中南大学湘雅二医院代谢内分泌研究所,长沙410011

出　　处：《广西医科大学学报》2012年第3期333-337,共5页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.30400218)

摘　　要：目的:观察前成骨样细胞(MC3T3-E1细胞)分化过程中17β-雌二醇对胰岛素受体底物-1(insulin receptor substrate-1,IRS-1)的影响。方法:应用10mmol/Lβ-甘油磷酸钠和50mg/L抗坏血酸诱导小鼠MC3T3-E1细胞分化成熟,分别在0,6,12,18,24,30d收集细胞。17β-雌二醇干预,用半定量RT-PCR和免疫印迹法检测干预组和对照组细胞IRS-1mRNA和蛋白的表达,比较两组细胞IRS-1表达量的变化。结果:在MC3T3-E1细胞分化成熟过程中,IRS-1 mRNA和蛋白的表达在MC3T3-E1细胞分化成熟过程中逐渐增高。结论:IRS-1可能在17β-雌二醇诱导的骨转换过程中起着重要作用。Objective： To observe the effect of estradiol on the expression profile of insulin receptor sub- strate-1 （IRS-1） during murine preosteoblastic MC3T3-E1 cells differentiation. Methods： MC3Td-E1 cell was cultured and underwent intervention of 17β-estradial. Cells were collected respectively in days 0, 6, 12, 18, 24 and 30. Semi-quantitative RT PCR was used to measure the expression of mRNA of IRS-1. The expression of the proteins of IRS-1 was detected by Western blotting. Results： IRS-1 mRNA and protein levels gradually increased during differentiation of MC3T3-E1 cells. Estradiol increased IRS-1 mRNA and protein expressions in the whole time course. Conclusion： IRS-1 may play an important role in the process of estradiol inducing bone turnover.

关 键 词：MC3T3-E1细胞 胰岛素受体底物-1 17Β-雌二醇 

分 类 号：R58[医药卫生—内分泌]