肉桂油抗柯萨奇病毒B_3的血清药理学研究  

Serum Pharmacological Study of Oleum Cinnamomi on Coxsackievirus B_3

作　　者：丁媛媛[1] 邱麟[2] 曾明[1] 许茜[1] 许景峰[1] 

机构地区：[1]北京军区总医院药理科,北京100700 [2]北京军区总医院口腔科,北京100700

出　　处：《医药导报》2012年第7期870-873,共4页Herald of Medicine

基　　金：国家自然科学基金资助项目(20872180)

摘　　要：目的利用血清药理学方法探讨肉桂油抗柯萨奇病毒B3(CVB3)的作用机制。方法制备SD大鼠乳鼠心肌炎细胞模型,72 h后,加入肉桂油静脉给药所得的大鼠含药血清至感染细胞模型,同时设立模型组与空白对照组,测定血清中不同时间点桂皮醛与肉桂酸的含量,并采用实时定量聚合酶链反应(PCR)法测定各组CVB3mRNA的相对含量,噻唑蓝(MTT)比色法测定各组心肌细胞存活率。采用SPSS统计学软件对其进行单因素相关性分析。结果肉桂油含药血清对心肌细胞存活率和CVB3mRNA的相对含量的影响与肉桂酸的浓度相关(P<0.05或P<0.01),而与桂皮醛的浓度无显著相关。结论肉桂油对病毒性心肌炎具有抗病毒活性,肉桂酸是肉桂油抗病毒的活性成分。Objective To investigate the effects and mechanism of oleum cinnamomi （OC） on primary rat myocardial cells infected with Coxsackie virus B3 （CVB3） by the serum pharmacological method. Methods The primary rat myocardial cells from the ventricle muscle of new born Sprague-Dawley rats were prepared. After 72 hours, different doses of OC contained sera obtained via i. v. injection with OC in rats were added in myocardial cells infected with CVB3 , to establish the model and control groups at the same time. The cinnamaldehyde and cinnamic acid in sera were detected at different time points. And the relative content of CVB3 mRNA was assayed by Real-time PCR. The survival rate of myocardial cells was determined through a metabolic 3-（ 4, 5-dimethyhhiazol-2-yl ）-2, 5-diphenyl-tetrazolium bromide （MTT） assay. A simple correlation test was implemented to analyze the relationship by using the SPSS software. Results The results showed that the effect of OC contained serum on the myocardial cell survival rate and relative amount of CVB3 mRNA was contributed to cinnamic acid P〈 0.05 or P〈0.01 ） rather than cinnamaldehyde. Conclusion Oleum cinnamon is an effective antiviral agent against viral myocarditis, the cinnamic acid is the main effective component for which.

关 键 词：肉桂油 桂皮醛 心肌炎 病毒性 血清药理学 

分 类 号：R285.5[医药卫生—中药学]