盐酸5-氨基酮戊酸脂质体的制备与表征  

Preparation and Characterization of Hydrochloric Acid 5-aminolevulinic Acid Liposomes

作　　者：吕玲[1,2] 刘宏[1] 喻晶[1] 罗远菊[1] 申玉莉[1] 王琪[1] 陈丹[1] 李珊[1] 

机构地区：[1]广州军区武汉总医院药剂科,武汉430070 [2]湖北中医药大学药学院硕士研究生班,武汉430065

出　　处：《医药导报》2012年第7期913-915,共3页Herald of Medicine

摘　　要：目的研究盐酸5-氨基酮戊酸(5-ALA)脂质体处方组成及制备工艺。方法采用薄膜分散-pH梯度法制备5-ALA脂质体,以包封率为评价指标进行正交实验筛选出最佳处方,采用高效液相色谱(HPLC)法测定其包封率。并对其粒径、电位等理化性质进行研究。结果最佳处方工艺为:卵磷脂与胆固醇的质量比为6∶1,卵磷脂与5-ALA质量比为6∶1,孵育温度为60℃。所制脂质体为乳白色,平均粒径为100 nm,Zeta电位为-40 mV,平均包封率为65.0%。结论该制备方法得到的5-ALA脂质体处方合理,工艺可行,包封率较高。Objective To study the formulation and preparation of hydrochloric acid 5-aminolevulinic acid （5-ALA） liposomes. Methods The 5-ALA lipsomes were produced by using film-pH gradient method evaluated by the encapsulation efficiency which was determined by HPLC. The prescription of 5-ALA was optimized by the orthogonal design. The physical and chemical properties as the particle size and electronic potential were studied. Results The optimum preparation technics were obtained as follows ： the ratio of lecithin to cholesterol was 6：1, lecithin to 5-ALA was 6 ： 1, which were incubated at 60 ~C. The liposomes produced was milk white, with an average particle size of 100 nm, Zeta potential as -40 mV, and the average encapsulation efficiency was 65.0%. Conclusion The formulation of 5-ALA liposomes is reasonable, the technology is practicable and efficient.

关 键 词：5-氨基酮戊酸 脂质体 制备工艺 

分 类 号：R979.14[医药卫生—药品] TQ460.6[医药卫生—药学]