小儿化毒散中芍药苷含量测定  

Content Determination of Paeoniflorin in Xiao'er Huadu Powder

作　　者：黄泽中[1] 何利华[2] 尹雄章[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,武汉430030 [2]湖北生态工程职业技术学院,武汉430068

出　　处：《医药导报》2012年第7期916-917,共2页Herald of Medicine

摘　　要：目的建立检测小儿化毒散中芍药苷含量的高效液相色谱法。方法色谱柱HiQ sil C18W(4.6 mm×250 mm,5μm),柱温:30℃,流动相:乙腈-0.1%磷酸(83∶17),流速:1.0 mL.min-1,紫外检测波长:230 nm。结果芍药苷含量在0.243～2.430μg范围内有良好线性关系,平均加样回收率100.1%,RSD=1.61%(n=6)。结论该方法简便,快速,准确,重复性好,可用于该制剂质量控制。Objective To develop a method for determination of paeoniflorin in xiao＇er huadu powder san by HPLC. Methods Chromatography was carried out on a column of HiQ sil C18 W（4.6 min×250 mm,5 μm） ,at 30 ℃. The mobile phase was composed of acetonitrile-0.1% phosphoric acid （83 ： 17 ）. The flow rate was 1.0 mL · min^-1 , and the detection wavelength was set at 230 nm. Results The content of paeoniflorin showed a good linearity in the range of 0. 243-2. 430μg and the average recovery was 100.1% ,with RSD as 1.61% （n=6）. Conclusion The method is simple,rapid,accurate,reproducible and can be used for the quality control of the preparation.

关 键 词：小儿化毒散 芍药苷 色谱法 高效液相 含量测定 

分 类 号：R286[医药卫生—中药学] R927.1[医药卫生—中医学]