小鼠哮喘模型中补体C5a对IL-17表达的调节作用  

Effects of complement C5a on IL-17 production in mouse asthma model

作　　者：肖燕[1] 谢晓伟[1] 龚琴[1] 文仲光[1] 

机构地区：[1]解放军总医院第一附属医院呼吸科,北京100048

出　　处：《临床肺科杂志》2012年第8期1361-1363,共3页Journal of Clinical Pulmonary Medicine

摘　　要：目的探讨小鼠哮喘模型中补体C5a对IL-17表达的调节作用。方法用卵清蛋白(OVA)致敏、激发的方法建立Balb/C小鼠哮喘模型。用体内抗体中和试验,观察致敏前中和炎症介质补体C5a的功能是否影响小鼠肺泡灌洗液中IL-17细胞因子的表达水平,并探讨其机制。结果病理结果显示模型组小鼠的肺组织出现肺泡腔扩张、腔内大量炎性细胞的浸润。小鼠致敏前给予抗C5a抗体干预上调了肺部IL-17及IL-6的表达,导致肺炎症明显加重。结论小鼠哮喘模型中体内C5a分子对IL-17表达具有负调节作用。Objective To explore the effects of complement C5a on IL-17 production in mice asthma model. Methods Asthma model were established in Balb/C mice by OVA immunization and challenge as described previously. Then neutralizing antibody were used to identify whether C5a blocking affect the production of IL-17 in vivo. Results OVA immunization and challenge resulted in increased in- flammatory response in the lung, which is marked by damaged pulmonary architecture and increased infiltration of the inflammatory cells. Neutralizing C5a before OVA immunization leads to up-regulated production of IL-17 and IL-6 in the lung. Conclusion In mouse asthma models, complement C5a act as regulatory roles in IL-17 production.

关 键 词：哮喘 IL—17 C5A 免疫调节 

分 类 号：R562.25[医药卫生—呼吸系统]