巴西橡胶树HbMyb1基因的原位PCR物理定位  

Chromosome Location of HbMyb1 Gene from Hevea brasiliensis by in situ PCR

作　　者：高和琼[1,2] 庄南生[2] 王英[2] 邱海燕[2] 

机构地区：[1]中国热带农业科学院橡胶研究所,海南儋州571737 [2]海南大学农学院,海南儋州571737

出　　处：《热带亚热带植物学报》2012年第4期365-368,共4页Journal of Tropical and Subtropical Botany

基　　金：国家天然橡胶产业技术体系(CARS-34);海南省自然科学基金项目(80658)资助

摘　　要：为探讨巴西橡胶树（Heveabrasiliensis）皮病抗性相关HbMyb]基因的定位，采用所建立的橡胶树染色体原位PCR技术体系进行研究。结果表明，HbMybl基因初步定位于巴西橡胶树‘热研7．33—97’的第5号染色体长臂上，信号位点到着丝粒的百分距离为15．21，并观察到在巴西橡胶树‘热研7．33．97’叶片细胞核的不同分裂时期均扩增到1～2个信号。同时对染色体标本的制备、保存、预处理等方面进行了探讨。The in situ PCR technical system on chromosomes of Hevea brasiliensis was developed successfully. The HbMybl gene related with Tapping Panel Dryness （TPD） resistance was physically located in the long arm of chromosome No. 5 of ＇Reyan 7-33-97＇ by in situ PCR and the fraction-length, the percentage of the distance from detection site to the centromere, was 15.21. One or two signals were detected during cell division at different phases. The chromosome preparations, preservation, and pretreatment were also discussed.

关 键 词：橡胶树 HbMyb1基因 原位PCR 染色体定位 

分 类 号：S794.1[农业科学—林木遗传育种]