木薯磷酸烯醇式丙酮酸羧化酶基因全长cDNA克隆及表达分析  

Cloning and Expression Analysis of Phosphoenolpyruvate Carboxylase Gene pepc from Cassava(Manihot esculenta Crantz)

作　　者：张杨[1] 陈新[2] 卢诚[2] 王文泉[2] 

机构地区：[1]海南大学农学院,海口571101 [2]中国热带农业科学院热带生物技术研究所,能源作物分子育种实验室,海口571101

出　　处：《热带亚热带植物学报》2012年第4期369-375,共7页Journal of Tropical and Subtropical Botany

基　　金：国家重点基础研究发展计划项目(2010CB126600)资助

摘　　要：应用巢式PCR从木薯栽培种(Manihot esculenta)Arg7和野生种W14(M.esculenta subsp.flabellifolia)叶片中克隆到磷酸烯醇式丙酮酸羧化酶基因pepc全长cDNA,GenBank序列号为JN387052和JN387053。cDNA全长2945 bp,含1个2895 bp的开放阅读框,预测编码的蛋白含964个氨基酸,具有磷酸烯醇式丙酮酸羧化酶保守结构域。木薯PEPC与麻疯树和蓖麻的PEPC氨基酸序列具有高度同源性。表达分析表明pepc在W14和Arg7叶中的表达量最高,其次是须根和块根,茎中最低。单日表达量动态分析表明,Arg7叶中pepc总体表达量高于W14,但是16∶00后W14高于Arg7,推测两种木薯pepc调控区域存在差异。The full-length cDNAs of phosphoenolpyruvate carboxylase gene pepc were cloned from cassava （Manihot esculenta） cultivar Arg7 and wild species （M. esculenta subsp, flabellifolia） W14 by using Nest-PCR method, respectively, with corresponding GenBank accession number of IN387053 and JN387052. Both of two cDNA sequences were 2945 bp in length including a ORF of 2895 bp, predicting to encode a protein with 964 amino acids and containing typical conserved sequences/domains of pepc gene. Cassava PEPC had high homologous with that in Ricinus communis and Jatropha curcas. The pepc expression was the highest in leaves of W14 and ArgT, followed fibrous roots and tuberous roots, and the lowest expression was in stems. The one- day dynamic expression analysis showed that the pepc expression in Arg7 leaves was higher than in W14 before 16： 00, while it was higher in W14 than Arg7 after 16： 00. Therefore, it suggested that the regulatory regions of pepc gene in two cassava species were different.

关 键 词：木薯 PEPC基因 生物信息学 

分 类 号：S533[农业科学—作物学]