上海四膜虫(Tetrahymena shanghaiensis)线粒体DNA的酶切分析  

Restriction Endonuclease Analysis of mtDNA of Tetrahymena shanghaiensis

作　　者：徐忠东[1] 刘家英[2] 庞延斌[2] 

机构地区：[1]安徽教育学院生物系,合肥230061 [2]华东师范大学生物系,上海200062

出　　处：《华东师范大学学报（自然科学版）》2000年第1期88-93,共6页Journal of East China Normal University(Natural Science)

摘　　要：该文以六种限制性内切酶对上海四膜虫的mtDNA进行单酶完全酶切 ,EcoRⅠ、HindⅢ、HhaⅠ酶切均产生 4个片段 ,PstⅠ、HpaⅡ、HaeⅢ酶切分别产生 5、6、7个片段。不同的酶切片段大小及总和有一定的差异 ,酶切位点分布不均匀 ,片段长度多态性明显。与其它四膜虫mtDNA的同种酶切结果比较 ,有较大的差异。同其它方法如四膜虫大核rDNA限制性内切酶分析、同工酶分析及皮层细胞骨架组分分析结果一致 ,进一步证明上海四膜虫是梨形四膜虫复合种的一种。mtDNA of Tetrahymena shanghaiensis (T. S1) was digested by six restriction endonucleases including: EcoRⅠ, HindⅢ, PstⅠ, HhaⅠ, HpaⅡ and HaeⅢ, respectively. The restriction sites of them were uneven. By contrast this result to same enzyme restriction maps of other mtDNA on Tetrahymena (Morin, 1988; Suyama, 1985; Norton, 1978). We found that the restriction maps of mtDNA of T. S1 was similar to those of T. pyr and was differed markedly from those of other Tetrahymena, The result was in accordance with those of the anlysis on the restriction digestion of rDNA and the research on four kinds of isozymes. It further demonstrated that T. S1 was a new species of Tetrahymena Pyriforms complex species.

关 键 词：上海四膜虫 MTDNA 酶切分析 线粒体DNA 

分 类 号：Q244[生物学—细胞生物学] Q959.1