人参和土壤中异菌脲残留分析  

Residual Analysis of Iprodione in Ginseng and Soil

作　　者：李月茹[1] 孙亮[1] 许煊炜[1] 

机构地区：[1]吉林农业大学农业部参茸产品质量监督检验测试中心,吉林长春130118

出　　处：《安徽农业科学》2012年第19期10359-10360,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立异菌脲在人参和土壤中的残留分析方法。[方法]人参和土壤样品分别用丙酮-石油醚和乙腈-水的混合溶剂提取、SPE柱净化,采用高效液相色谱法(HPLC)测定人参和土壤中的异菌脲残留量。[结果]在0.04～5.00 mg/kg浓度范围内,异菌脲浓度(x)与峰面积(y)的线性回归方程为:y=42.495x-0.674,r=0.999 7,线性关系良好。异菌脲在土壤和人参中的添加回收率分别为96.60%～100.80%和92.90%～105.70%,变异系数分别为1.54%～2.78%和7.20%～9.50%。异菌脲在土壤和人参中的最小检出量均为3.0×10-10g,实际土壤、人参添加异菌脲的定量限分别为0.04和0.09 mg/kg。[结论]该方法准确、快速、灵敏度高,能够满足农药残留分析要求。[Objective] The aim was to establish an analysis method for iprodione residues in ginseng and soil.[Method] Ginseng and soil samples were respectively extracted with a mixture of acetone and petroleum ether and a mixture of acetonitrile and water,and then cleaned up by a SPE column.The residues of iprodione in ginseng and soil samples were determined by HPLC.[Result] The linear regression equation between iprodione concentration（0.04-5.00 mg/kg） and peak area was y = 42.495x-0.674,and r=0.999 7,which suggested a good linear relation.The fortified recoveries of iprodione in soil and ginseng were 96.60%-100.80% and 92.90%-105.70%,and the coefficients of variation were 1.54%-2.78% and 7.20%-9.50%,respectively.The LOD（limit of detection） of iprodione in soil and ginseng were both 3.0×10-10 g,and the LOQ（limit of quantification） iprodione in soil and ginseng were 0.04 and 0.09 mg/kg,respectively.[Conclusion] The method is accurate and fast,and it could meet requirements for pesticide residue analysis.

关 键 词：异菌脲 人参 土壤 残留分析 

分 类 号：S481.8[农业科学—农药学]