正交法优选厚朴叶中厚朴酚与和厚朴酚的提取工艺  

Optimization of Extraction Technology of Magnolol and Honokiol from the Leaves of Magnolia officinalis Rehd.et Wils. through Orthogonal Test

作　　者：张丹[1] 颜学伟[1] 王刚[1] 文小峰[1] 李雪梅[1] 罗丹[1] 曹纬国[1,2,3] 

机构地区：[1]重庆医科大学中医药学院,重庆401331 [2]重庆医科大学中医药研究室,重庆400016 [3]重庆医科大学中医药实验教学中心,重庆401331

出　　处：《安徽农业科学》2012年第21期10810-10811,10836,共3页Journal of Anhui Agricultural Sciences

基　　金：重庆市九龙坡区科技项目(2011-62-1);重庆市渝中区科技创新项目(20100208)

摘　　要：[目的]优选厚朴叶中厚朴酚与和厚朴酚的提取工艺条件。[方法]以厚朴酚与和厚朴酚的总提取率为考察指标,采用正交试验法对厚朴叶中厚朴酚与和厚朴酚的提取条件进行优选。[结果]最佳提取条件为:料液比1∶10(g/ml),乙醇浓度80%,提取次数1次,提取时间1.5 h。[结论]该提取工艺方法合理,有效成分提取效率较高,适用于厚朴叶中活性成分的提取。[Objective] To optimize the extraction technology of magnolol and honokiol from the leaf of Magnolia officinalis Rehd.et Wils.[Method] Taking the extraction rate of magnolol and honokiol as evaluation index,the extraction technology of magnolol and honokiol from M.officinalis leaves was optimized by means of orthogonal test.[Result] The optimal extraction technology was as follows： M.officinalis leaves were extracted once with 80% ethanol at the solid-liquid ratio of 1∶10 g/ml for 1.5 h.[Conclusion] The extraction technology was reasonable and effective to extract effective components including magnolol and honokiol from M.officinalis leaves.

关 键 词：厚朴叶 厚朴酚 和厚朴酚 正交试验 HPLC 

分 类 号：S567[农业科学—中草药栽培]