怀山药零余子薯蓣皂苷元成分的含量测定  

Determination of the Diosgenin Content of Dioscorea opposita Thunb. and Bulbil

作　　者：滕井通[1] 高翔[1] 薛建平[1] 李紫薇[1] 张爱民[1] 盛玮[1] 

机构地区：[1]淮北师范大学生命科学学院,安徽淮北235000

出　　处：《安徽农业科学》2012年第21期10817-10818,10877,共3页Journal of Anhui Agricultural Sciences

基　　金：资源植物生物学安徽省重点实验室项目

摘　　要：[目的]测定怀山药及其零余子中薯蓣皂苷元成分的含量。[方法]以齐墩果酸为标准对照品,浓度5%香草醛-冰醋酸和高氯酸混合液为显色剂,用紫外可见分光光度计在波长547 nm处对怀山药及其零余子的皂苷成分进行含量测定。[结果]怀山药中薯蓣皂苷元含量为0.016 4%,怀山药零余子中薯蓣皂苷元含量为0.021 3%,平均加标回收率为98.46%,RSD为1.36%;零余子中薯蓣皂苷元含量略高于怀山药。[结论]该方法简便、易行、准确度高,可用于怀山药零余子中薯蓣皂苷元成分的含量测定。[Objective] to determine the diosgenin content of Dioscorea opposita Thunb.and bulbil.[Method] With 5% vanillin-glacial acetic acid-perchloric acid as color developing agent and oleanolic acid as the control substance,the content of diosgenin in D.opposita and bulbil was measured using an ultraviolet visible spectrophotometer at the detection wavelength of 547 nm.[Result] The content of diosgenin in D.opposita and bulbil was 0.016 4% and 0.021 3%,respectively.The average recovery of diosgenin was 98.46% with relative standard deviations（RSD） of 1.36%.Besides,the content of diosgenin in bulbil was slightly higher than that in D.opposita.[Conclusion] The method is simple,easy,accurate,and suitable for content determination of diosgenin in D.opposita and bulbil.

关 键 词：怀山药(Dioscorea opposita Thunb.) 零余子 皂苷 比色法 

分 类 号：S567[农业科学—中草药栽培]