纳米银对原代培养海马神经细胞凋亡的研究  

Study on the Apoptosis of Rat Hippocampal Neurons in Primary Culture due to Silver Nanoparticles

作　　者：王燕 张伟 李恺 滕安娜[2] 王辉 徐五通 刘元敏 顾希 

机构地区：[1]东营出入境检验检疫局,山东东营257091 [2]泰山职业技术学院,山东泰安271000

出　　处：《安徽农业科学》2012年第21期10896-10897,10921,共3页Journal of Anhui Agricultural Sciences

基　　金：山东出入境检验检疫局科研项目(SK201151)

摘　　要：[目的]探讨纳米银对原代培养海马神经细胞的影响。[方法]用吖啶橙染色观察细胞形态及染色质的变化,用Fluo3荧光染料检测细胞内游离Ca2+浓度变化,并利用ELLSA试剂盒检测Caspase-3凋亡酶活性。[结果]与正常对照组相比,经50μg/ml纳米银处理36h后,海马神经细胞的凋亡率达到最高((48.3±0.33)%),凋亡酶活性增强,并且细胞内游离的Ca2+荧光强度达到41.70±18.78。结论50μg/ml纳米银作用36 h可导致海马神经元凋亡。[Objective] To discuss the effect of silver nanoparticles on the apoptosis of rat hippocampal neurons in primary culture.[Method] The morphology and chromatin of cells were observed with acridine orange（AO） staining,the changes of Ca2＋ concentration was measured with Fluo3,and the caspase-3 activities were determined with opticaldelnsity（OD）.[Result] Compared with normal rat hippocampal neurons,the apoptotic ratio of hippocampal neurons reached the maximum of（48.3±0.33）% after being treated with 50 μg/ml silver nanoparticles for 36 h.The caspase-3 activity was enhanced,and the average fluorescence of Ca2＋ reached to 41.70±18.78.[Conclusion] Treated with 50 μg/ml silver nanoparticles for 36 h,the cells underwent apoptotic processes.

关 键 词：纳米银 海马神经元 凋亡 CA2+ Caspase-3凋亡酶 

分 类 号：S609.9[农业科学—园艺学]