Nodal基因5′末端侧翼序列启动子的分析  

Analysis of the Mouse Nodal Gene Promoter on Its 5' Flanking Sequence

作　　者：胡新立[1] 周迅蕾[1] 

机构地区：[1]北京大学生命科学学院,北京100871

出　　处：《北京大学学报（自然科学版）》2000年第2期178-185,共8页Acta Scientiarum Naturalium Universitatis Pekinensis

基　　金：国家自然科学基金!(39470371) ;国家教委留学回国人员科研启动费资助

摘　　要：为了鉴定Ｎｏｄａｌ 基因的基本启动子元件，将Ｎｏｄａｌ 基因５′侧翼序列的各缺失片段构建以荧光素酶为报告基因的重组质粒。用这些携带报告基因的质粒转化Ｆ９ 细胞并测定了它们的瞬时表达荧光素酶活性。Ｎｏｄａｌ 基因的基本启动子元件位于转录起始位点上游约６０ ｂｐ，其中含有一个位于－ ３３ ｂｐ 处的ＴＡＴＡｂｏｘ，它对于转录起始起着重要作用。这个基本启动子在多种细胞类型中都有活性，但其活性不强。Nodal gene is critical to mouse development.It plays a vital role in the mesoderm formation and also has a biological function during mouse embryo pattern formation. To determine the basal promoter,the 5′ flanking sequence of Nodal gene were truncated and inserted into the luciferase reported vector. These constructs were transiently transfected into F?9 cells and the luciferase activity of each construct was determined. The basal promoter was located at about 60?bp up stream of the transcription initiation site. It contains a TATA box at -33?bp which is required for the transcription initiation. This basal promoter is active in many cell types,but its activity is not very strong.

关 键 词：NODAL基因 表达调控 启动子 侧翼序列 

分 类 号：Q344.14[生物学—遗传学]