葱蝇谷胱甘肽S-转移酶基因的克隆与序列分析  

Cloning and Sequence Analysis of Glutathione S-transferase Gene in Delia antiqua

作　　者：夏洁[1] 陈斌[1] 郝友进[1] 司风铃[1] 

机构地区：[1]重庆师范大学昆虫与分子生物学研究所重庆高校生物活性物质工程研究中心重庆市动物生物学重点实验室,重庆401331

出　　处：《重庆师范大学学报（自然科学版）》2012年第4期24-28,共5页Journal of Chongqing Normal University:Natural Science

基　　金：国家自然科学基金(No.30870340);重庆市教委科学技术研究项目(No.KJ100620);重庆师范大学校级基金重点项目(No.2011XLZ12)

摘　　要：谷胱苷肽S-转移酶(GST)是昆虫体内广泛存在的一类解毒酶,可以保护机体免受内源性或氧化物的损害。昆虫对一些杀虫剂的抗性与GST表达水平增高有关。GST的研究主要集中在杀虫剂抗性上,可将其作为昆虫的药物靶标,设计和开发新型的杀虫剂。采用RACE的方法,克隆了葱蝇(Delia antiqua)GST基因cDNA的全长序列(GenBank登录号:JQ625502),获得的cDNA全长874bp,其中阅读框ORF 627bp,编码208个氨基酸,推测其相对分子质量为23.9kD,等电点为5.83。通过该基因推导的氨基酸序列与其它物种的GST蛋白进行相似性比较和系统发育分析,发现葱蝇与丝光绿蝇(Lucilia cuprina)的谷胱甘肽转移酶氨基酸序列同源性最高。该结果进一步丰富了GST基因的基础数据,有助于葱蝇的杀虫剂抗性机理和发育机理的相关研究。Glutathione S transferase （GST） is a kind of detoxifying enzymes widely existing in insects. Its peroxidase vitality can protect the organisms against endogenous or oxide damage. The insect resistance to some pesticides is relative to the expression quantity of GST, and past research of insect GST mainly focused on the important role in the formation of pesticides resist ance. This kind of genes can be used as the target of insecticide to design and develop new pesticides. In this research, the full length of GST cDNA of the onion maggot （Delia antiqua） was cloned using RACE technique （GenBank access number： JQ625502）. The result showed that the full length of cDNA is 874 bp long, with an open reading frame （ORF） of 627 bp, en coding a protein of 208 amino acids with a calculated molecular weight of 23.9 kD and theoretical isolectric point of 5.83. The deduced amino acid sequence has the highest identity with that of Lucilia cuprina based homological analysis, and a phylogenic tree was inferred with homological GST sequences from other insects. The results provide a base and information frame for fur- ther research of the GST gene, and contribute to the related research of the insecticide resistance mechanism and development mechanism about Delia antiqua

关 键 词：葱蝇 谷胱甘肽S-转移酶 GST基因 序列分析 系统发育树 

分 类 号：Q966[生物学—昆虫学] Q785