水稻线粒体nad5基因超表达载体的构建和遗传转化  

Construction and genetic transformation of over-expression vector of nad5 genes in rice

作　　者：冷翔 谭艳平[1] 王春台[1] 刘学群[1] 

机构地区：[1]中南民族大学生命科学院/生物技术国家民委重点实验室,湖北武汉430074

出　　处：《广东农业科学》2012年第12期1-2,7,F0002,共4页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31170296)

摘　　要：以TRIzol法提取的水稻幼苗总RNA反转录的cDNA为模板,以重组PCR技术扩增得到nad5的ORF,构建含有线粒体信号肽Rf1b5'的nad5双元超表达载体pCAMBIA1305.1::35S::Rf1b5'::nad5,以农杆菌介导的愈伤组织侵染法进行水稻遗传转化。克隆得到的水稻nad5基因大小为2 013 bp,构建了携带线粒体信号肽Rf1b5'的nad5植物表达载体,并成功获得了超表达nad5基因的阳性植株。The ORF of nad5 was amplified with the template cDNA reverse transcripted from the total RNA in rice seedlings through recombinant PCR.The recombinant binary expression vector pCAMBIA1305.1::35S::Rf1b5'::nad5 was constructed through linking the ORF of nad5 and Rf1b5.Through Agrobacterium-mediated approach the recombinant plasmid was transformed into the rice callus.The size of gene nad5 in rice was 2 013 bp.The recombinant binary expression vector pCAMBIA1305.1::35S::Rf1b5'::nad5 was constructed successfully.A lot of transgenic plants over-expressed nad5 were obtained.

关 键 词：水稻 nad5基因 遗传转化 

分 类 号：Q244[生物学—细胞生物学]